Construct: ORF TRCN0000474048
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007121.1_s317c1
- Derived from:
- ccsbBroadEn_09976
- DNA Barcode:
- CAATTTGGGCATCGCGCACCGAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IGSF10 (285313)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474048
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285313 | IGSF10 | immunoglobulin superfamily ... | NM_001178146.2 | 99.8% | 99.8% | 528T>G;665A>G;1620A>G |
| 2 | human | 285313 | IGSF10 | immunoglobulin superfamily ... | NM_001178145.2 | 92.4% | 92.4% | (many diffs) |
| 3 | human | 285313 | IGSF10 | immunoglobulin superfamily ... | NM_178822.4 | 22.9% | 22.9% | (many diffs) |
| 4 | human | 285313 | IGSF10 | immunoglobulin superfamily ... | XM_011512709.2 | 22.9% | 22.9% | (many diffs) |
| 5 | human | 285313 | IGSF10 | immunoglobulin superfamily ... | XM_011512708.2 | 22.3% | 22.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1872
- ORF length:
- 1806
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ggatgatctg atactgatgc atgttagcct aagactgaaa cctgccaaaa 121 ttgaccacaa gcagtatttt agaaagcaag tgctccatgg gaaagatttc caagtagatt 181 gcaaagcttc cggctcccca gtgccagaga tatcttggag tttgcctgat ggaaccatga 241 tcaacaatgc aatgcaagcc gatgacagtg gccacaggac taggagatat acccttttca 301 acaatggaac tttatacttc aacaaagttg gggtagcgga ggaaggagat tatacttgct 361 atgcccagaa caccctaggg aaagatgaaa tgaaggtcca cttaacagtt ataacagctg 421 ctccccggat aaggcagagt aacaaaacca acaagagaat caaagctgga gacacagctg 481 tccttgactg tgaggtcact ggggatccca aaccaaaaat attttggttg ctgccttcca 541 atgacatgat ttccttctcc attgataggt acacatttca tgccaatggg tcgttgacca 601 tcaacaaagt gaaactgctc gattctggag agtacgtatg tgtagcccga aatcccagtg 661 gggatgacac caaaatgtac aaactggatg tggtctctaa acctccatta atcaatggtc 721 tgtatacaag cagaactgtt attaaagcca cagctgtgag acattccaaa aaacactttg 781 actgcagagc tgaagggaca ccatctcctg aagtcatgtg gatcatgcca gacaatattt 841 tcctcacagc cccatactat ggaagcagaa tcacagtcca taaaaatgga accttggaaa 901 ttaggaatgt gaggctttca gattcagccg actttatctg tgtggcccga aatgaaggtg 961 gagagagcgt gttggtagta cagttagaag tactggaaat gctgagaaga ccgacattta 1021 gaaatccatt taatgaaaaa atagttgccc agctgggaaa gtccacagca ttgaattgct 1081 ctgttgatgg taacccacca cctgaaataa tctggatttt accaaatggc acacgatttt 1141 ccaatggacc acaaagttat cagtatctga tagcaagcaa tggttctttt atcatttcta 1201 aaacaactcg ggaggatgca ggaaaatatc gctgtgcagc taggaataaa gttggctata 1261 ttgagaaatt agtcatatta gaaattggcc agaagccagt tattcttacc tatgcaccag 1321 ggacagtaaa aggcatcagt ggagaatctc tatcactgca ttgtgtgtct gatggaatcc 1381 ctaagccaaa tatcaaatgg actatgccaa gtggttatgt agtagacagg cctcaaatta 1441 atgggaaata catattgcat gacaatggca ccttagtcat taaagaagca acagcttatg 1501 acagaggaaa CTATATCTGT AAGGCTCAAA ATAGTGTTGG TCATACACTG ATTACTGTTC 1561 CAGTAATGAT TGTAGCCTAC CCTCCCCGAA TTACAAATCG TCCACCCAGG AGTATTGTCA 1621 CCAGGACAGG GGCAGCCTTT CAGCTCCACT GTGTGGCCTT GGGAGTTCCC AAGCCAGAAA 1681 TCACGTGGGA GATGCCTGAC CACTCCCTTC TCTCAACGGC AAGTAAAGAG AGGACACATG 1741 GAAGTGAGCA GCTTCACTTA CAAGGTACCC TAGTCATTCA GAATCCCCAA ACCTCCGATT 1801 CTGGGATATA CAAATGCACA GCAAAGAACC CACTTGGTAG TGATTATGCA GCAACGTATA 1861 TTCAAGTAAT CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1921 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1981 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACAA TTTGGGCATC GCGCACCGAA 2041 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t