Construct: ORF TRCN0000474053
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005071.1_s317c1
- Derived from:
- ccsbBroadEn_01309
- DNA Barcode:
- AGTGAAAGAATCACCCCTATGAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSMB1 (5689)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474053
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5689 | PSMB1 | proteasome 20S subunit beta 1 | NM_002793.4 | 100% | 100% | |
| 2 | mouse | 19170 | Psmb1 | proteasome (prosome, macrop... | NM_011185.3 | 85.3% | 92.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 789
- ORF length:
- 723
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt gtcctctaca gccatgtatt cggctcctgg cagagacttg gggatggaac 121 cgcacagagc cgcgggccct ttgcagctgc gattttcgcc ctacgttttc aacggaggta 181 ctatactggc aattgctgga gaagattttg caattgttgc ttctgatact cgattgagtg 241 aagggttttc aattcatacg cgggatagcc ccaaatgtta caaattaaca gacaaaacag 301 tcattggatg cagcggtttt catggagact gtcttacgct gacaaagatt attgaagcaa 361 gactaaagat gtataagcat tccaataata aggccatgac tacgggggca attgctgcaa 421 tgctgtctac aatcctgtat tcaaggcgct tctttccata ctatgtttac aacatcatcg 481 gtggacttga tgaagaagga aagggggctg tatacagctt tgatccagta gggtcttacc 541 agagagactc cttcaaggct ggaggctcag caagtgccat gctacagccc ctgcttgaca 601 accaggttgg ttttaagaac atgcagaatg tggagcatgt tccgctgtcc ttggacagag 661 ccatgcggct ggtgaaagat gtcttcattt cTGCGGCTGA GAGAGATGTG TACACTGGGG 721 ACGCACTCCG GATCTGCATA GTGACCAAAG AGGGCATCAG GGAGGAAACT GTTTCCTTAA 781 GGAAGGACTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 841 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 901 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGTGAA AGAATCACCC CTATGAACAC 961 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt