Construct: ORF TRCN0000474064
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003542.1_s317c1
- Derived from:
- ccsbBroadEn_05200
- DNA Barcode:
- GTGATTTTAAACTCCCGATAAACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMIM14 (201895)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474064
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 201895 | SMIM14 | small integral membrane pro... | NM_001317896.2 | 100% | 100% | |
| 2 | human | 201895 | SMIM14 | small integral membrane pro... | NM_001317897.2 | 100% | 100% | |
| 3 | human | 201895 | SMIM14 | small integral membrane pro... | NM_174921.3 | 100% | 100% | |
| 4 | mouse | 68552 | Smim14 | small integral membrane pro... | NM_133697.3 | 82.4% | 89.8% | (many diffs) |
| 5 | mouse | 68552 | Smim14 | small integral membrane pro... | XM_006504083.2 | 82.4% | 89.8% | (many diffs) |
| 6 | mouse | 68552 | Smim14 | small integral membrane pro... | XM_006504084.3 | 82.4% | 89.8% | (many diffs) |
| 7 | mouse | 68552 | Smim14 | small integral membrane pro... | XM_006504085.3 | 82.4% | 89.8% | (many diffs) |
| 8 | mouse | 68552 | Smim14 | small integral membrane pro... | XM_006504086.3 | 82.4% | 89.8% | (many diffs) |
| 9 | mouse | 68552 | Smim14 | small integral membrane pro... | XM_006504087.3 | 82.4% | 89.8% | (many diffs) |
| 10 | mouse | 68552 | Smim14 | small integral membrane pro... | XM_006504088.3 | 82.4% | 89.8% | (many diffs) |
| 11 | mouse | 68552 | Smim14 | small integral membrane pro... | XM_006504089.2 | 82.4% | 89.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 240
- ORF end:
- 537
- ORF length:
- 297
- Sequence:
-
1 TCGTCTTTAG GTTGGGGGGA GGGGTTTTAT GCGATGGAGT TTCCCCCACA CTGAGTGGGT 61 GGAGACTGAA GTTAGGCCAG CTTGGCACTT GATGTAATTC TCCTTGGAAT TTGCCCTTTT 121 TGAGTTTGGA TCTTGGTTCA TTCTCAAGCC TCAGACAGTG GTTCAAAGTT TTTTTCTTCC 181 ATTTCAGGTG TCGTGAGGCT AGCATCGATT GATCAACAAG TTTGTACAAA AAAGTTGGCA 241 TGGCAGAAGG TGGATTTGAT CCCTGTGAAT GTGTTTGCTC TCATGAACAT GCAATGAGAA 301 GACTGATCAA TCTGTTACGG CAGTCCCAGT CCTACTGCAC AGACACAGAG TGTCTTCAGG 361 AATTACCGGG ACCCTCTGGT GATAATGGCA TCAGTGTTAC AATGATCTTG GTAGCCTGGA 421 TGGTTATTGC ATTGATCTTG TTCTTACTGA GACCTCCTAA TCTAAGAGGA TCCAGCCTAC 481 CTGGAAAGCC AACCAGTCCT CATAATGGAC AAGATCCACC AGCTCCTCCT GTGGACTACC 541 TAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 601 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 661 ATATATCTTG TGGAAAGGAC GAGTGATTTT AAACTCCCGA TAAACTACGC GTTAAGTCga 721 caatcaacct ctggattaca aaatttgtga aagatt