Construct: ORF TRCN0000474065
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011260.1_s317c1
- Derived from:
- ccsbBroadEn_15737
- DNA Barcode:
- CCAGGAACCAGCGAGACGCACCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DIDO1 (11083)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474065
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11083 | DIDO1 | death inducer-obliterator 1 | NM_001193369.2 | 21.3% | 21.2% | 1_5286del;6043G>C |
| 2 | human | 11083 | DIDO1 | death inducer-obliterator 1 | NM_033081.2 | 21.3% | 21.2% | 1_5286del;6043G>C |
| 3 | human | 11083 | DIDO1 | death inducer-obliterator 1 | XM_011528509.2 | 21.3% | 21.2% | 1_5286del;6043G>C |
| 4 | human | 11083 | DIDO1 | death inducer-obliterator 1 | XM_011528504.1 | 20.9% | 20.9% | 1_5394del;6151G>C |
| 5 | human | 11083 | DIDO1 | death inducer-obliterator 1 | XM_011528505.2 | 20.9% | 20.9% | 1_5394del;6151G>C |
| 6 | human | 11083 | DIDO1 | death inducer-obliterator 1 | XM_011528507.1 | 20.9% | 20.9% | 1_5394del;6151G>C |
| 7 | human | 11083 | DIDO1 | death inducer-obliterator 1 | XM_011528508.1 | 20.9% | 20.9% | 1_5394del;6151G>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1500
- ORF length:
- 1434
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc agggcttcac ggccccaatt tcccgggacc aagggggcca gcccctccgt 121 ttccagaaga gaatatcgct tctaacgatg ggccacgagg gcctccgcca gccagattcg 181 gagcccagaa ggggcccatc ccttccttat tctcggggca acatgggcca cctccttatg 241 gggacagcag aggcccctca ccctcttacc ttggtggacc acgaggagtg gcaccatccc 301 aatttgaaga acgcaaggat ccccatgggg agaagaggga gttccaggac gccccgtata 361 acgaggtgac gggcgccccc gcccagtttg aagggacaga gcaagcccca tttctgggaa 421 gcagaggcgg cgcgcctttc cagttcggag gccagagaag gccactgctg tctcagctga 481 aaggcccccg aggcggcccc cctccctctc agtttggagg tcagagagga ccaccccctg 541 gtcatttcgt gggcccaaga gggccccatc ctagtcagtt tgaaactgcc cggggccctc 601 atcccaacca gtttgaagga cccagaggcc aagcgcctaa ctttatgcca ggtcccaggg 661 gcattcagcc tcagcagttc gaagaccaga gggtccattc accaccaaga ttcacaaacc 721 aaagggcgcc tgcacccctg cagtttggtg gactacgggg gtccgcaccc ttttctgaaa 781 aaaatgagca gaccccttcg cgatttcact tccagggcca gcccccgcag gtgatgaagc 841 cgggccccag gcccctgctg gagcttccca gccacccccc gcagcaccgg aaggaccgct 901 gggaggaggc cgggccgccc tccgcgctct cctccagtgc gcccggacag ggccccgagg 961 ccgacggaca gtgggcatcg gccGACTTCC GAGAGGGGAA AGGCCACGAA TACAGAAACC 1021 AGACTTTCGA AGGGAGGCAG AGAGAGCGGT TTGACGTGGG GCCCAAAGAG AAGCCGCTGG 1081 AGGAGCCCGA CGCCCAGGGC CGGGCGTCCG AGGACAGGAG GAGAGAGCGC GAGCGCGGCC 1141 GAAACTGGAG CCGAGAGCGG GACTGGGACC GGCCCCGGGA GTGGGACCGA CACCGGGACA 1201 AGGACTCCAG CCGGGACTGG GACAGAAACC GGGAGAGGAG CGCCAACCGC GACCGAGAGC 1261 GCGAGGCCGA CCGGGGCAAG GAGTGGGACC GCAGCCGGGA GCGGAGCAGG AACCGAGAGC 1321 GCGAGCGAGA CCGGAGGCGC GACCGGGACC GGTCCCGGAG CAGAGAGCGG GACCGAGACA 1381 AGGCCAGGGA CAGGGAGCGG GGCCGCGACC GCAAGGACCG GAGCAAGAGC AAAGAGAGCG 1441 CTCGGGACCC GAAGCCCGAG GCCTCGAGGG CCTCCGACGC TGGCACCGCC TCGCAGGCCT 1501 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1561 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1621 TTTATATATC TTGTGGAAAG GACGACCAGG AACCAGCGAG ACGCACCCGA CGCGTTAAGT 1681 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt