Transcript: Human NM_033081.2

Homo sapiens death inducer-obliterator 1 (DIDO1), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-08-02
Taxon:
Homo sapiens (human)
Gene:
DIDO1 (11083)
Length:
8592
CDS:
357..7079

Additional Resources:

NCBI RefSeq record:
NM_033081.2
NBCI Gene record:
DIDO1 (11083)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_033081.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000262646 TCGCCGTCACTGTTGTATAAA pLKO_005 1923 CDS 100% 15.000 21.000 N DIDO1 n/a
2 TRCN0000262645 CCAACGCCCTGTATTGCATTT pLKO_005 1153 CDS 100% 10.800 15.120 N DIDO1 n/a
3 TRCN0000015093 GCGAGCGATCACAATTACAAT pLKO.1 1872 CDS 100% 5.625 7.875 N DIDO1 n/a
4 TRCN0000282358 CGGTGCTCAGGCAGGTATTAA pLKO_005 1721 CDS 100% 15.000 12.000 N DIDO1 n/a
5 TRCN0000015094 GCCTCACAACAACAGGTTTAT pLKO.1 1181 CDS 100% 13.200 9.240 N DIDO1 n/a
6 TRCN0000015097 GCAGATCAGCAGGAAGCTAAA pLKO.1 1353 CDS 100% 10.800 7.560 N DIDO1 n/a
7 TRCN0000015095 CCAAGGGATAAAGGGTAGAAT pLKO.1 1445 CDS 100% 5.625 3.938 N DIDO1 n/a
8 TRCN0000074350 CCTCATCCCAACCAGTTTGAA pLKO.1 6174 CDS 100% 5.625 3.938 N DIDO1 n/a
9 TRCN0000074352 ACGAATACAGAAACCAGACTT pLKO.1 6583 CDS 100% 4.950 3.465 N DIDO1 n/a
10 TRCN0000194338 CAGCCTCACAACAACAGGTTT pLKO.1 1179 CDS 100% 4.950 3.465 N Dido1 n/a
11 TRCN0000074349 CGAATACAGAAACCAGACTTT pLKO.1 6584 CDS 100% 4.950 3.465 N DIDO1 n/a
12 TRCN0000074348 CCACGATCTTAGATTCAGGAT pLKO.1 7154 3UTR 100% 2.640 1.848 N DIDO1 n/a
13 TRCN0000074351 CAGAAGAGAATATCGCTTCTA pLKO.1 5701 CDS 100% 0.495 0.347 N DIDO1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_033081.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02618 pDONR223 100% 24.7% 23.8% None (many diffs) n/a
2 ccsbBroad304_02618 pLX_304 0% 24.7% 23.8% V5 (many diffs) n/a
3 TRCN0000468117 ATCGTACAAGACCGAACAACTTTA pLX_317 25.9% 24.7% 23.8% V5 (many diffs) n/a
4 ccsbBroadEn_11591 pDONR223 100% 23.8% 23.6% None (many diffs) n/a
5 ccsbBroad304_11591 pLX_304 0% 23.8% 23.6% V5 (many diffs) n/a
6 TRCN0000474152 CATGTTCATTACTTCGACCCTCAA pLX_317 16.5% 23.8% 23.6% V5 (many diffs) n/a
7 ccsbBroadEn_15737 pDONR223 0% 21.3% 21.2% None 1_5286del;6043G>C n/a
8 ccsbBroad304_15737 pLX_304 0% 21.3% 21.2% V5 1_5286del;6043G>C n/a
9 TRCN0000474065 CCAGGAACCAGCGAGACGCACCCG pLX_317 36.2% 21.3% 21.2% V5 1_5286del;6043G>C n/a
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