Construct: ORF TRCN0000474070
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003760.1_s317c1
- Derived from:
- ccsbBroadEn_09590
- DNA Barcode:
- AGTCCACGCACTTCCCATCACCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZFP28 (140612)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474070
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 140612 | ZFP28 | ZFP28 zinc finger protein | NM_020828.2 | 99.9% | 99.7% | 119C>T;128C>G |
| 2 | human | 140612 | ZFP28 | ZFP28 zinc finger protein | XM_011526463.3 | 94% | 92.4% | (many diffs) |
| 3 | human | 140612 | ZFP28 | ZFP28 zinc finger protein | XM_011526462.3 | 88.8% | 88.8% | 0_1ins291 |
| 4 | human | 140612 | ZFP28 | ZFP28 zinc finger protein | NM_001308440.1 | 37.6% | 35.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2673
- ORF length:
- 2604
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcggggggcg gcgagcgcga gtgtccgcga gccgacgccg ctcccgggta 121 gaggcgcccc ccgcacaaag ccccgggcgg gccgaggccc gactgtaggg actccagcca 181 ccttggtcct ccctggccgg ggaaggccgc gctcaaggaa tggcctcgca tccaaaggcc 241 agcgaggagc ggcccctacg gggcctgggc acagagctct gccttccagg gacactgctc 301 ttccccagga gagaaacaag aagctggagg ctgtggggac aggaattgaa cctaaagcca 361 tgtcccaggg cttggtgaca tttggggatg tggctgtaga tttctcccaa gaggagtggg 421 agtggctgaa ccccattcag aggaacttgt acaggaaggt gatgttggag aactacagga 481 acctggcatc gctgggactt tgtgtttcta agcccgatgt gatctcctcg ttggaacaag 541 gaaaagagcc ttggacagtg aagcgaaaga tgacaagagc ctggtgccca gacttgaagg 601 ctgtgtggaa gatcaaggag ttacctctca agaaggactt ctgcgaagga aagctatccc 661 aggcagtgat aacagagaga ctcacaagct ataatctgga gtactctctg ttaggggaac 721 actgggatta tgatgctctg tttgagacac agccgggctt ggtgactatc aaaaacctgg 781 ctgttgactt ccgccagcag ctacacccag ctcagaagaa tttctgtaag aatgggatat 841 gggagaacaa cagtgacctg ggatcagcag gacattgtgt ggctaagcca gatttagtct 901 ctttactaga gcaagagaag gagccctgga tggtgaagcg agagctgaca ggaagcctgt 961 tctcaggcca gcgatctgta catgagaccc aggaattatt tccaaagcaa gattcatatg 1021 ctgaaggggt aacagacaga acctcaaaca ctaaacttga ttgttccagt ttcagagaaa 1081 attgggattc tgactatgtg tttggaagga agcttgcagt aggtcaagag acacaattca 1141 ggcaagagcc aattactcat aacaaaaccc tctctaagga aagagaacgt acatataaca 1201 aatctggaag atggttctat ttggacgatt cagaagagaa agttcataat cgtgattcaa 1261 ttaaaaattt tcaaaaaagt tcagtggtaa taaaacaaac aggcatctat gcaggaaaaa 1321 agcttttcaa gtgtaatgaa tgtaagaaaa cttttaccca gagctcatct cttactgttc 1381 atcagagaat tcacactgga gagaaacctt ataaatgtaa tgaatgtggg aaggccttta 1441 gtgacggctc atcctttgcc cgacaccaga gatgtcacac tggcaagaag ccctatgagt 1501 gcattgagtg tgggaaagct ttcatacaga acacatccct tatccgtcac tggagatact 1561 atcatactgg ggagaaaccc tttgattgca tcgattgtgg gaaagccttc agtgaccaca 1621 tagggcttaa tcaacacagg agaattcata ctggagagaa accttacaaa tgtgatgtat 1681 gtcacaaatc cttcaggtat ggttcctccc ttactgtaca tcaaaggatt cataccggag 1741 aaaaaccata tgaatgtgat gtttgcagaa aagccttcag ccatcatgca tcactcactc 1801 aacatcaaag agtacattct ggagaaaagc cttttaagtg taaagagtgc ggaaaagctt 1861 ttaggcagaa tatacacctt gccagtcatt taaggattca tactggggag aagccttttg 1921 aatgtgcgga gtgtggaaaa tccttcagca tcagttctca gcttgccact catcagagaa 1981 tccatactgg agagaagccc tatgaatgta aggtttgtag taaagcgttc acccagaagg 2041 ctcaccttgc acagcatcag aaaacccata caggagagaa accatatgag tgcaaggaat 2101 gcggtaaagc cttcagccag accacacacc tcattcaaca tcagagagtt cacactggtg 2161 agaaacccta taaatgtatg gaatgtggga aggcctttgg tgataactca tcctgtactc 2221 aacatcaaag actgcacact ggccaaagac cttatgaatg tattgagtgt ggaaaggcat 2281 tcaagacaaa atcctccctt atttgtcatc gcagaagtca tactggagaa aaaccttatg 2341 aatgcagtgt gtgtggcaaa gcctttagtc atcgtcaatc ccttagtgta catcagagaa 2401 tccattctgg aaagaaacca tatgaatgta aggaatgtag gaaaaccttc atccaaattg 2461 gacaccttaa tcaacataag agagttcata ctggagagag atcttataac tataagaaaa 2521 gcagaaaagt cttcaggcaa actgctcact tagctcatca tcagcgaatt catactggag 2581 agtcgtcaac atgcccctct ttaccttcca cgtcaaatcc tgtGGATCTG TTTCCCAAAT 2641 TTCTCTGGAA TCCATCCTCC CTCCCATCAC CATTGCCAAC TTTCTTGTAC AAAGTGGTTG 2701 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2761 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAG 2821 TCCACGCACT TCCCATCACC ATACGCGTTA AGTCgacaat caacctctgg attacaaaat 2881 ttgtgaaaga tt