Construct: ORF TRCN0000474074
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013903.1_s317c1
- Derived from:
- ccsbBroadEn_08987
- DNA Barcode:
- TCTCAATATACGGCGGTAAGTGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NANOG (79923)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474074
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 388112 | NANOGP8 | Nanog homeobox retrogene P8 | NM_001355281.1 | 99.8% | 99.6% | 47A>C |
| 2 | human | 79923 | NANOG | Nanog homeobox | NM_024865.4 | 99.5% | 99.3% | (many diffs) |
| 3 | human | 79923 | NANOG | Nanog homeobox | NM_001297698.2 | 94.4% | 94% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 984
- ORF length:
- 915
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagtgtggat ccagcttgtc cccaaagctt gccttgcttt gaagcatccg 121 actgtaaaga atcttcacct atgcctgtga tttgtgggcc tgaagaaaac tatccatcct 181 tgcaaatgtc ttctgctgag atgcctcaca cagagactgt ctctcctctt ccttcctcca 241 tggatctgct tattcaggac agccctgatt cttccaccag tcccaaaggc aaacaaccca 301 cttctgcaga gaatagtgtc gcaaaaaagg aagacaaggt cccggtcaag aaacagaaga 361 ccagaactgt gttctcttcc acccagctgt gtgtactcaa tgatagattt cagagacaga 421 aatacctcag cctccagcag atgcaagaac tctccaacat cctgaacctc agctacaaac 481 aggtgaagac ctggttccag aaccagagaa tgaaatctaa gaggtggcag aaaaacaact 541 ggccgaagaa tagcaatggt gtgacgcaga aggccTCAGC ACCTACCTAC CCCAGCCTCT 601 ACTCTTCCTA CCACCAGGGA TGCCTGGTGA ACCCGACTGG GAACCTTCCA ATGTGGAGCA 661 ACCAGACCTG GAACAATTCA ACCTGGAGCA ACCAGACCCA GAACATCCAG TCCTGGAGCA 721 ACCACTCCTG GAACACTCAG ACCTGGTGCA CCCAATCCTG GAACAATCAG GCCTGGAACA 781 GTCCCTTCTA TAACTGTGGA GAGGAATCTC TGCAGTCCTG CATGCACTTC CAGCCAAATT 841 CTCCTGCCAG TGACTTGGAG GCTGCCTTGG AAGCTGCTGG GGAAGGCCTT AATGTAATAC 901 AGCAGACCAC TAGGTATTTT AGTACTCCAC AAACCATGGA TTTATTCCTA AACTACTCCA 961 TGAACATGCA ACCTGAAGAC GTGTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1021 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1081 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT CTCAATATAC 1141 GGCGGTAAGT GCGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1201 att