Construct: ORF TRCN0000474084
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003178.1_s317c1
- Derived from:
- ccsbBroadEn_03867
- DNA Barcode:
- TACTTACACATCATTTATTACCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SINHCAF (58516)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474084
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 58516 | SINHCAF | SIN3-HDAC complex associate... | NM_001135811.1 | 100% | 100% | |
| 2 | human | 58516 | SINHCAF | SIN3-HDAC complex associate... | NM_001135812.2 | 100% | 100% | |
| 3 | human | 58516 | SINHCAF | SIN3-HDAC complex associate... | NM_021238.2 | 100% | 100% | |
| 4 | human | 58516 | SINHCAF | SIN3-HDAC complex associate... | XM_017019758.2 | 100% | 100% | |
| 5 | human | 58516 | SINHCAF | SIN3-HDAC complex associate... | XM_017019759.2 | 100% | 100% | |
| 6 | human | 58516 | SINHCAF | SIN3-HDAC complex associate... | XM_017019760.1 | 100% | 100% | |
| 7 | human | 58516 | SINHCAF | SIN3-HDAC complex associate... | XM_017019761.1 | 100% | 100% | |
| 8 | human | 58516 | SINHCAF | SIN3-HDAC complex associate... | XM_017019762.2 | 100% | 100% | |
| 9 | human | 58516 | SINHCAF | SIN3-HDAC complex associate... | XM_011520803.2 | 67.3% | 67.3% | 1_321del |
| 10 | mouse | 56306 | Fam60a | family with sequence simila... | NM_019643.3 | 92.6% | 97.7% | (many diffs) |
| 11 | mouse | 56306 | Fam60a | family with sequence simila... | XM_006507074.3 | 92.6% | 97.7% | (many diffs) |
| 12 | mouse | 56306 | Fam60a | family with sequence simila... | XM_006507075.2 | 92.6% | 97.7% | (many diffs) |
| 13 | mouse | 56306 | Fam60a | family with sequence simila... | XM_006507073.3 | 58.1% | 61.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 729
- ORF length:
- 663
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt tggttttcac aagccaaaga tgtaccgaag tatagagggc tgctgtattt 121 gcagagctaa gtcctccagt tctcgattca ctgacagtaa acgctatgaa aaggacttcc 181 agagctgttt tggattgcat gagactcgtt caggagacat ctgcaatgcc tgtgtcctgc 241 ttgtgaaaag atggaagaag ttgccagcag gatcaaaaaa aaactGGAAT CATGTGGTAG 301 ATGCAAGGGC TGGACCCAGT CTAAAGACTA CATTGAAACC AAAGAAAGTG AAAACTCTAT 361 CTGGGAACAG GATAAAAAGC AACCAGATCA GTAAACTGCA GAAGGAATTT AAACGTCATA 421 ATTCTGATGC TCACAGTACC ACCTCAAGTG CCTCCCCAGC TCAATCTCCT TGTTACAGTA 481 ACCAGTCAGA TGACGGCTCA GATACAGAGA TGGCTTCTGG TTCTAACAGA ACACCAGTTT 541 TTTCCTTTTT AGATCTCACT TACTGGAAAA GACAGAAGAT ATGTTGTGGG ATCATCTATA 601 AAGGCCGTTT TGGGGAAGTC CTCATTGACA CACATCTCTT CAAGCCTTGC TGCAGCAATA 661 AGAAAGCAGC TGCTGAGAAG CCAGAGGAGC AGGGGCCAGA GCCTCTGCCC ATCTCCACTC 721 AGGAGTGGTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 781 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 841 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATACTTA CACATCATTT ATTACCTAAC 901 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt