Transcript: Mouse XM_006507074.3

PREDICTED: Mus musculus family with sequence similarity 60, member A (Fam60a), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Fam60a (56306)
Length:
2603
CDS:
124..789

Additional Resources:

NCBI RefSeq record:
XM_006507074.3
NBCI Gene record:
Fam60a (56306)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006507074.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000240922 GTTGTGGGATCATCTATAAAG pLKO_005 641 CDS 100% 13.200 18.480 N Fam60a n/a
2 TRCN0000202419 GAAGTCCTCATCGACACTCAT pLKO.1 673 CDS 100% 4.950 6.930 N Fam60a n/a
3 TRCN0000240921 ATACGTGGCTGCCTTAGTATT pLKO_005 2057 3UTR 100% 13.200 10.560 N Fam60a n/a
4 TRCN0000263879 TATGTTGTGGGATCATCTATA pLKO_005 638 CDS 100% 13.200 10.560 N SINHCAF n/a
5 TRCN0000240919 TCCCGGTTCACGGACAGTAAA pLKO_005 199 CDS 100% 13.200 10.560 N Fam60a n/a
6 TRCN0000215880 CCTAGTCTAAAGACAACATTG pLKO.1 373 CDS 100% 10.800 8.640 N Fam60a n/a
7 TRCN0000240923 AGAGCAGGCCCTAGTCTAAAG pLKO_005 364 CDS 100% 10.800 7.560 N Fam60a n/a
8 TRCN0000189644 CCACAGTTTGTGGCATCCTAT pLKO.1 2146 3UTR 100% 4.950 3.465 N Fam60a n/a
9 TRCN0000240920 AGTGGTGACTGAGGTTCATTT pLKO_005 782 CDS 100% 13.200 7.920 N Fam60a n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006507074.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03867 pDONR223 100% 92.6% 97.7% None (many diffs) n/a
2 ccsbBroad304_03867 pLX_304 0% 92.6% 97.7% V5 (many diffs) n/a
3 TRCN0000474084 TACTTACACATCATTTATTACCTA pLX_317 66.4% 92.6% 97.7% V5 (many diffs) n/a
4 ccsbBroadEn_12419 pDONR223 100% 30.3% 31.6% None (many diffs) n/a
5 ccsbBroad304_12419 pLX_304 0% 30.3% 31.6% V5 (many diffs) n/a
6 TRCN0000466073 AATTCCCCCCCCAGACCGACGGGC pLX_317 100% 30.3% 31.6% V5 (many diffs) n/a
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