Construct: ORF TRCN0000474096
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017305.3_s317c1
- Derived from:
- ccsbBroadEn_15026
- DNA Barcode:
- CAAAGCTTAGATGTAGCCTTTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DSTYK (25778)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474096
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25778 | DSTYK | dual serine/threonine and t... | NM_199462.3 | 99.9% | 99.8% | 1921T>C |
| 2 | human | 25778 | DSTYK | dual serine/threonine and t... | NM_015375.3 | 95.1% | 95% | 1921T>C;2467_2601del |
| 3 | human | 25778 | DSTYK | dual serine/threonine and t... | XM_011509392.2 | 94.1% | 94% | 625_626ins27;1894T>C;2440_2574del |
| 4 | human | 25778 | DSTYK | dual serine/threonine and t... | XM_011509393.2 | 72.8% | 71.9% | (many diffs) |
| 5 | human | 25778 | DSTYK | dual serine/threonine and t... | XM_011509394.2 | 72.1% | 71.5% | (many diffs) |
| 6 | mouse | 213452 | Dstyk | dual serine/threonine and t... | NM_172516.4 | 84.1% | 86.6% | (many diffs) |
| 7 | mouse | 213452 | Dstyk | dual serine/threonine and t... | XM_006529356.3 | 74.7% | 77.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2718
- ORF length:
- 2652
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gggcgacggg gtgccatggg gcagcgagcc cgtctcgggt cccggccccg 121 gcggcggcgg aatgatccgc gagctgtgcc ggggcttcgg ccgctaccgc cgctacctgg 181 gacggctgcg acagaacctg cgcgagaccc agaagttctt ccgcgacatc aagtgctccc 241 acaaccacac ttgtctctcc tccctcacgg gcggcggcgg ggccgagcgc ggccctgcag 301 gcgatgtcgc cgaaaccggg ctgcaggcgg gccaactgag ctgcatttcc ttcccaccta 361 aggaagagaa gtacctccag cagattgtgg actgcctccc ttgcatactg atcctcggcc 421 aggattgtaa cgtcaagtgc cagctgttga atctgctgtt gggggtgcag gtgcttccca 481 ccaccaagct gggcagtgag gagagctgta agcttcggcg cctccgcttc acctatggga 541 ctcagactcg ggtcagcctg gcgctccctg gacagtatga actagtgcac acgctggttg 601 ctcatcaggg caactgggag accatccctg aggaggatct ggaggtccaa gagaacaatg 661 aggatgctgc tcatgtttta gcggaactgg aggtaacgat gcaccatgct ctcttacagg 721 aagtggacgt tgtggtagca ccatgccaag gcctccggcc cacagtggat gttctgggtg 781 acttggtgaa tgatttcttg cctgtgataa cctatgcact ccacaaagat gaactctctg 841 agagggatga gcaagagctt caggaaatcc gaaagtattt ctcctttcct gtattctttt 901 tcaaagtgcc gaaactgggc tcggagataa tagactcctc aaccaggaga atggagagcg 961 aaagatcacc gctttatcgc cagctaattg acctgggcta tctgagcagc agtcactgga 1021 actgtggggc tcctggccag gatactaaag ctcagagcat gttggtggaa cagagtgaaa 1081 agctgagaca cttgagcaca ttttctcacc aggtgttaca gactcgcctg gtggatgcag 1141 ccaaggccct gaacctggtg cactgccact gccttgacat ctttattaac caggcatttg 1201 acatgcagcg ggacctgcag atcactccca aacgtctgga atatactcga aaaaaggaga 1261 atgagttgta tgaatcattg atgaatattg ccaaccgaaa gcaggaggaa atgaaggata 1321 tgattgttga gacacttaat accatgaagg aggaacttct ggatgatgct actaacatgg 1381 agtttaaaga cgtcattgtc cctgagaatg gagaaccagt aggcaccaga gagatcaaat 1441 gctgcatccg acagatccag gaactcatca tctcccgact taatcaggca gtggctaata 1501 agctgatcag ctcagtggat tacctgaggg aaagcttcgt cggaaccctg gaacgatgtc 1561 tgcagagcct ggagaagtct caggatgtct cagttcacat caccagtaat tatctcaaac 1621 agatcttaaa tgctgcctat catgttgaag tcacgtttca ctcagggtcg tcagttacaa 1681 ggatgctatg ggagcaaatc aaacagatca tccagcgcat cacatgggtg agcccacctg 1741 ccatcactct ggaatggaag aggaaggtgg cccaggaagc cattgagagc ctcagcgcct 1801 ccaaattggc taagagcatt tgcagccaat tccggactcg gctcaatagt tcccacgagg 1861 cttttgcagc ctccttgcgg cagctggaag ctggccactc aggccggtta gagaaaacgg 1921 aagatctatg gctgagggtt cggaaagatc atgctccccg cctggcccgc ctttctctgg 1981 aaagccgttc tttacaggat gtcttgcttc atcgtaaacc taaactggga caggaactgg 2041 gccggggcca gtatggtgtg gtatacctgt gtgacaactg gggaggacac ttcccttgtg 2101 ccctcaaatc agttgtccct ccagatgaga agcactggaa tgatctggct ttggaatttc 2161 actatatgag gtctctgccg aagcatgagc gattggtgga tctccatggt tcagtcattg 2221 actacaacta tggtggtggc tccagcattg ctgtgcTCCT CATTATGGAG CGGCTACACC 2281 GGGATCTCTA CACAGGGCTG AAGGCTGGGC TGACCCTGGA GACACGTTTG CAGATAGCAC 2341 TAGATGTGGT GGAGGGAATC CGCTTCCTGC ACAGCCAGGG ACTTGTCCAT CGTGATATCA 2401 AACTGAAAAA TGTGCTGCTG GATAAGCAGA ACCGTGCCAA GATCACTGAC TTAGGATTCT 2461 GCAAGCCAGA GGCCATGATG TCAGGCAGCA TTGTGGGGAC ACCAATCCAT ATGGCCCCTG 2521 AACTTTTCAC AGGGGCTCGC CCAGAACGTC TTCCTGTGTT TGATGAGGAG TGCTGGCAGT 2581 TGATGGAAGC CTGTTGGGAT GGCGACCCCT TGAAGAGGCC TCTCTTGGGC ATTGTCCAGC 2641 CCATGCTCCA GGGCATCATG AATCGGCTCT GCAAGTCCAA TTCTGAGCAG CCAAACAGAG 2701 GACTAGATGA TTCTACTTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2761 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2821 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACAAAGCT TAGATGTAGC 2881 CTTTTTCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt