Construct: ORF TRCN0000474102
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002333.1_s317c1
- Derived from:
- ccsbBroadEn_04233
- DNA Barcode:
- GCCCAGTTGCTACTTTCGATTTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CFHR5 (81494)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474102
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81494 | CFHR5 | complement factor H related 5 | NM_030787.4 | 100% | 100% | |
| 2 | human | 81494 | CFHR5 | complement factor H related 5 | XM_011510020.2 | 97.6% | 94.5% | (many diffs) |
| 3 | human | 3080 | CFHR2 | complement factor H related 2 | NM_005666.4 | 39.4% | 34.6% | (many diffs) |
| 4 | human | 3080 | CFHR2 | complement factor H related 2 | XM_011509458.2 | 38.9% | 34.6% | (many diffs) |
| 5 | human | 3080 | CFHR2 | complement factor H related 2 | XM_011509459.2 | 36.4% | 32.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1776
- ORF length:
- 1707
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttgctctta ttcagtgtaa tcctaatctc atgggtatcc actgttgggg 121 gagaaggaac actttgtgat tttccaaaaa tacaccatgg atttctgtat gatgaagaag 181 attataaccc tttttcccaa gttcctacag gggaagtttt ctattactcc tgtgaatata 241 attttgtgtc tccttcaaaa tccttttgga ctcgcataac atgcacagaa gaaggatggt 301 caccaacacc gaagtgtctc agaatgtgtt cctttccttt tgtgaaaaat ggtcattctg 361 aatcttcagg actaatacat ctggaaggtg atactgtaca aattatttgc aacacaggat 421 acagccttca aaacaatgag aaaaacattt cgtgtgtaga acggggctgg tccactcctc 481 ccatatgcag cttcactaaa ggagaatgtc atgttccaat tttagaagcc aatgtagatg 541 ctcagccaaa aaaagaaagc tacaaagttg gagacgtgtt gaaattctcc tgcagaaaaa 601 atcttataag agttggatca gactcagttc aatgttacca atttgggtgg tcacctaact 661 ttccaacatg caaaggacaa gtacgatcat gtggtccacc tcctcaactc tccaatggtg 721 aagttaagga gataagaaaa gaggaatatg gacacaatga agtagtggaa tatgattgca 781 atcctaattt tataataaac gggcctaaga aaatacaatg tgtggatgga gaatggacaa 841 ctttacccac ttgtgttgaa caagtgaaaa catgtggata catacctgaa ctcgagtacg 901 gttatgttca gccgtctgtc cctccctatc aacatggagt ttcagtcgag gtgaattgca 961 gaaatgaata tgcaatgatt ggaaataaca tgattacctg tattaatgga atatggacag 1021 agcttcctat gtgtgttgca acacaccaac ttaagaggtg caaaatagca ggagttaata 1081 taaaaacatt actcaagcta tctgggaaag aatttaatca taattctaga atacgttaca 1141 gatgttcaga catcttcaga tacaggcact cagtctgtat aaacgggaaa tggaatcctg 1201 aagtagactg cacagaaaaa agggaacaat tctgcccacc gccacctcag atacctaatg 1261 ctcagaatat gacaaccaca gtgaattatc aggatggaga aaaagtagct gttctctgta 1321 aagaaaacta tctacttcca gaagcaaaag aaattgtatg taaagatgga cgatggcaat 1381 cattaccacg ctgtgttgag tctactgcat attgtgGGCC CCCTCCATCT ATTAACAATG 1441 GAGATACCAC CTCATTCCCA TTATCAGTAT ATCCTCCAGG GTCAACAGTG ACGTACCGTT 1501 GCCAGTCCTT CTATAAACTC CAGGGCTCTG TAACTGTAAC ATGCAGAAAT AAACAGTGGT 1561 CAGAACCACC AAGATGCCTA GATCCATGTG TGGTATCTGA AGAAAACATG AACAAAAATA 1621 ACATACAGTT AAAATGGAGA AACGATGGAA AACTCTATGC AAAAACAGGG GATGCTGTTG 1681 AATTCCAGTG TAAATTCCCA CATAAAGCGA TGATATCATC ACCACCATTT CGAGCAATCT 1741 GTCAGGAAGG GAAATTTGAA TATCCTATAT GTGAATTGCC AACTTTCTTG TACAAAGTGG 1801 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1861 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1921 AGCCCAGTTG CTACTTTCGA TTTCCACGCG TTAAGTCgac aatcaacctc tggattacaa 1981 aatttgtgaa agatt