Construct: ORF TRCN0000474105
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012517.1_s317c1
- Derived from:
- ccsbBroadEn_14049
- DNA Barcode:
- ACATTCCCCCCTCAATTCTAAAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CIB2 (10518)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474105
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10518 | CIB2 | calcium and integrin bindin... | NM_001271888.2 | 99.7% | 100% | 264G>A |
| 2 | human | 10518 | CIB2 | calcium and integrin bindin... | XM_006720374.2 | 99.7% | 100% | 264G>A |
| 3 | human | 10518 | CIB2 | calcium and integrin bindin... | NM_001271889.2 | 91.9% | 85.4% | (many diffs) |
| 4 | human | 10518 | CIB2 | calcium and integrin bindin... | XM_011521161.2 | 83% | 83.2% | (many diffs) |
| 5 | human | 10518 | CIB2 | calcium and integrin bindin... | NM_006383.4 | 76.8% | 77% | 1_129del;393G>A |
| 6 | human | 10518 | CIB2 | calcium and integrin bindin... | NM_001301224.2 | 69.4% | 66.1% | (many diffs) |
| 7 | human | 10518 | CIB2 | calcium and integrin bindin... | XM_005254126.3 | 66.2% | 66.1% | (many diffs) |
| 8 | human | 10518 | CIB2 | calcium and integrin bindin... | NR_125435.2 | 26.9% | (many diffs) | |
| 9 | human | 10518 | CIB2 | calcium and integrin bindin... | XR_001751051.1 | 20.4% | (many diffs) | |
| 10 | mouse | 56506 | Cib2 | calcium and integrin bindin... | NM_019686.5 | 68.6% | 74.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 501
- ORF length:
- 432
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggactacagg aagagcccca tcgtccacgt gcccatgagc ctcatcatcc 121 agatgccaga gctccgggag aatcccttca aagaaaggat cgtggcggcg ttttccgagg 181 atggtgaggg gaacctcact ttcaacgact ttgtggacat gttttccgtg ctcTGCGAGT 241 CGGCTCCCCG AGAGCTCAAG GCAAACTATG CCTTCAAGAT CTATGACTTC AACACTGACA 301 ACTTCATCTG CAAGGAGGAC CTGGAGCTGA CACTGGCCCG GCTCACTAAG TCAGAGCTGG 361 ATGAGGAGGA GGTGGTGCTT GTGTGCGACA AGGTCATTGA GGAGGCTGAC TTGGACGGTG 421 ACGGCAAGCT GGGCTTTGCT GACTTCGAGG ACATGATTGC CAAGGCCCCT GACTTCCTCA 481 GCACTTTCCA CATCCGGATC TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 541 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 601 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACAT TCCCCCCTCA 661 ATTCTAAAAC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt