Construct: ORF TRCN0000474109
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001448.1_s317c1
- Derived from:
- ccsbBroadEn_05409
- DNA Barcode:
- TTTCTCAGGTAGGCTTATACGCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL7L1 (285855)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474109
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285855 | RPL7L1 | ribosomal protein L7 like 1 | NM_001366481.2 | 96.4% | 96.4% | 1_27del |
| 2 | human | 285855 | RPL7L1 | ribosomal protein L7 like 1 | NM_198486.4 | 96.4% | 96.4% | 1_27del |
| 3 | human | 285855 | RPL7L1 | ribosomal protein L7 like 1 | NR_134563.2 | 19% | (many diffs) | |
| 4 | human | 285855 | RPL7L1 | ribosomal protein L7 like 1 | NR_134562.2 | 18.6% | 1_322del;441_556del;1177_3960del | |
| 5 | mouse | 66229 | Rpl7l1 | ribosomal protein L7-like 1 | NM_025433.3 | 84.6% | 83.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 804
- ORF length:
- 738
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggagcaagag caaagaaaaa tccctttggt tccagaaaat ctcctgaaaa 121 agaggaaggc ttatcaagcc ctcaaagcca cccaggcaaa gcaggcactt ttggcaaaga 181 aggagcagaa gaaaggaaaa gggctcaggt ttaagcgact ggaatcattc ctacatgatt 241 cctggcggca gaaacgtgac aaggtgcgtc tcagacgact agaagtgaaa cctcatgcct 301 tggaattgcc agataaacat tccttggcct ttgttgtacg catcgaaagg attgacggcg 361 tgagtttact ggtgcagaga accattgcaa gacttcgcct aaagaaaatt tttagtggTG 421 TCTTTGTAAA AGTCACCCCC CAGAATCTAA AAATGCTGCG TATAGTGGAA CCTTATGTGA 481 CCTGGGGATT TCCAAATCTG AAGTCTGTCC GAGAACTCAT TTTGAAACGT GGACAAGCCA 541 AGGTCAAGAA TAAGACCATC CCTCTGACAG ACAATACAGT GATTGAGGAG CACCTGGGGA 601 AGTTTGGCGT CATTTGCTTG GAAGACCTCA TTCATGAAAT TGCCTTCCCA GGGAAGCATT 661 TCCAGGAGAT CTCATGGTTC TTGTGCCCTT TCCACCTCTC AGTGGCCCGT CATGCTACCA 721 AAAATAGAGT GGGCTTCCTC AAGGAGATGG GCACACCTGG CTATCGGGGT GAACGCATCA 781 ATCAGCTCAT CCGTCAGCTG AACTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 841 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 901 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TTCTCAGGTA 961 GGCTTATACG CCGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1021 att