Construct: ORF TRCN0000474118
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013712.1_s317c1
- Derived from:
- ccsbBroadEn_03465
- DNA Barcode:
- ACTGGCCACTTGAAGTTTGTTCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GDAP2 (54834)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474118
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54834 | GDAP2 | ganglioside induced differe... | NM_001135589.3 | 100% | 100% | |
| 2 | human | 54834 | GDAP2 | ganglioside induced differe... | NM_017686.4 | 98.1% | 97.3% | (many diffs) |
| 3 | mouse | 14547 | Gdap2 | ganglioside-induced differe... | NM_010269.3 | 86.8% | 92.4% | (many diffs) |
| 4 | mouse | 14547 | Gdap2 | ganglioside-induced differe... | XM_006501001.2 | 86.8% | 92.4% | (many diffs) |
| 5 | mouse | 14547 | Gdap2 | ganglioside-induced differe... | XR_001783651.1 | 49.8% | (many diffs) | |
| 6 | mouse | 14547 | Gdap2 | ganglioside-induced differe... | NR_104341.1 | 43.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1554
- ORF length:
- 1488
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tcccttaggt gcaccttccc agtttgtgga tgtggataca ctaccaagct 121 ggggtgactc atgccaagat gaattaaatt cctctgatac tacagctgaa atatttcagg 181 aagacactgt tcgatcacct tttctttata ataaggacgt caatggaaaa gtggttcttt 241 ggaaaggaga tgtggcatta ctgaactgta cagccattgt gaataccagc aatgaaagtc 301 tcacagataa gaatcctgtg tcagaaagta tcttcatgct tgcagggcct gatttgaagg 361 aagatctcca gaaacttaaa gggtgccgaa caggtgaagc aaaattgaca aaaggattca 421 atctagctgc ccggttcatc attcacacag tgggacctaa atataaaagc cgctatcgca 481 cagcagctga gagttccctt tatagctgct acagaaacgt acttcaacta gcaaaagagc 541 agtcaatgtc ttctgttggc ttctgtgtca tcaattctgc aaaacgtggt tatcctttag 601 aggatgcaac acacatagca cttcgcactg taagaagatt cctagagatt catggggaaa 661 ccattgaaaa agtagtattt gctgtctctg atcttgaaga gggtacttac caaaagctgc 721 tacctctcta cttcccaagg tcattaaaag aggagaatcg atcattgccc tacctacctg 781 cagatattgg aaatgcagaa ggggagcctg tggtacctga acgacagatt agaataagtg 841 agaaacctgg tgctccagaa gataaccaag aagaggagga tgaaggcttg ggagttgatc 901 tctctttcat tggctctcat gcttttgctc gaatggaagg agatattgac aagcaaagaa 961 aactgatcct tcagggacaa ttatcagagg cagctctgca gaagcagcat caaagaaatt 1021 ataatcgctg gttatgtcaa gcaagatctg aggatctgtc tgatattgct tctctaaaag 1081 ccttatacca aacaggtgtt gataactgtg gtcgaacagt gatggtggta gttGGAAGAA 1141 ACATTCCTGT AACATTAATA GATATGGACA AGGCTCTCTT ATATTTCATT CATGTAATGG 1201 ATCACATTGC TGTGAAGGAG TATGTATTAG TGTATTTTCA CACCCTGACC AGCGAATACA 1261 ATCACCTGGA CTCCGACTTC CTGAAGAAAC TCTACGATGT TGTTGATGTC AAGTACAAGA 1321 GGAATTTGAA GGCTGTTTAT TTTGTACATC CCACATTTCG TTCAAAGGTG TCAACATGGT 1381 TTTTTACCAC CTTTTCTGTC TCAGGACTGA AGGACAAAAT CCACCATGTG GACAGCCTCC 1441 ACCAGCTGTT TTCTGCCATA TCACCAGAAC AGATTGACTT TCCTCCTTTT GTCCTTGAAT 1501 ATGATGCCAG GGTAAGAAGT ACCAGAAGTT CTCCCTCACC TGGTATGGTA TATTACCCAA 1561 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1621 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1681 TATCTTGTGG AAAGGACGAA CTGGCCACTT GAAGTTTGTT CGAACGCGTT AAGTCgacaa 1741 tcaacctctg gattacaaaa tttgtgaaag att