Construct: ORF TRCN0000474120
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006914.1_s317c1
- Derived from:
- ccsbBroadEn_01352
- DNA Barcode:
- CAATGAGTATTAATGCGAAAAACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PEX2 (5828)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474120
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5828 | PEX2 | peroxisomal biogenesis fact... | NM_001079867.1 | 100% | 100% | |
| 2 | human | 5828 | PEX2 | peroxisomal biogenesis fact... | NM_001172086.1 | 100% | 100% | |
| 3 | human | 5828 | PEX2 | peroxisomal biogenesis fact... | NM_001172087.1 | 100% | 100% | |
| 4 | human | 5828 | PEX2 | peroxisomal biogenesis fact... | NM_000318.3 | 99.8% | 99.6% | 550T>C |
| 5 | mouse | 19302 | Pex2 | peroxisomal biogenesis fact... | NM_001163301.2 | 86.6% | 88.1% | (many diffs) |
| 6 | mouse | 19302 | Pex2 | peroxisomal biogenesis fact... | NM_001163302.2 | 86.6% | 88.1% | (many diffs) |
| 7 | mouse | 19302 | Pex2 | peroxisomal biogenesis fact... | NM_001163305.2 | 86.6% | 88.1% | (many diffs) |
| 8 | mouse | 19302 | Pex2 | peroxisomal biogenesis fact... | NM_001163306.2 | 86.6% | 88.1% | (many diffs) |
| 9 | mouse | 19302 | Pex2 | peroxisomal biogenesis fact... | NM_001267714.1 | 86.6% | 88.1% | (many diffs) |
| 10 | mouse | 19302 | Pex2 | peroxisomal biogenesis fact... | NM_001267715.1 | 86.6% | 88.1% | (many diffs) |
| 11 | mouse | 19302 | Pex2 | peroxisomal biogenesis fact... | NM_008994.4 | 86.6% | 88.1% | (many diffs) |
| 12 | mouse | 19302 | Pex2 | peroxisomal biogenesis fact... | XM_006530058.3 | 86.6% | 88.1% | (many diffs) |
| 13 | mouse | 19302 | Pex2 | peroxisomal biogenesis fact... | XM_006530059.2 | 86.6% | 88.1% | (many diffs) |
| 14 | mouse | 19302 | Pex2 | peroxisomal biogenesis fact... | XM_011248140.2 | 86.6% | 88.1% | (many diffs) |
| 15 | mouse | 19302 | Pex2 | peroxisomal biogenesis fact... | XM_017319505.1 | 86.6% | 88.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 981
- ORF length:
- 915
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttccagaaaa gagaatgcga agagtgcaaa cagagtgcta agaataagcc 121 agttggatgc acttgaacta aacaaggccc tggagcagct agtttggtcc cagtttactc 181 agtgctttca tggatttaaa cctgggctgt tagctcgctt tgagccagag gtgaaagcgt 241 gcttatgggt tttcttgtgg agattcacca tctactccaa aaatgccaca gtgggacagt 301 cagttttgaa tattaagtac aaaaatgatt tttcccctaa cctgagatat cagccaccca 361 gtaaaaatca aaaaatctgg tatgctgttt gtacaattgg tggcaggtgg ttagaagaac 421 gatgctatga tttgtttcga aaccatcatt tagcatcatt tgggaaagtc aagcagtgtg 481 tgaattttgt gattggactt ttgaaattag gtgggctgat taattttttg attttccttc 541 agaggggaaa gtttgcaact ttgacagaac gtcTCCTAGG TATTCATTCT GTATTTTGCA 601 AGCCTCAAAA CATACGTGAA GTTGGCTTTG AATACATGAA TAGGGAACTT CTCTGGCATG 661 GTTTTGCTGA ATTTCTGATT TTTCTCTTAC CACTTATCAA TGTCCAGAAG TTGAAAGCCA 721 AGCTGTCTTC ATGGTGTATT CCTCTTACTG GTGCACCTAA TAGTGACAAT ACATTAGCCA 781 CCAGTGGCAA AGAATGCGCT CTATGTGGAG AGTGGCCCAC CATGCCTCAC ACCATAGGAT 841 GTGAGCATAT TTTCTGTTAT TTCTGTGCTA AGAGTAGTTT CTTATTTGAC GTGTACTTTA 901 CTTGTCCTAA GTGTGGCACA GAAGTACACA GTCTGCAGCC ACTGAAATCA GGAATCGAGA 961 TGTCAGAAGT AAATGCTCTT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1021 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1081 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACAAT GAGTATTAAT 1141 GCGAAAAACC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt