Construct: ORF TRCN0000474122
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003986.1_s317c1
- Derived from:
- ccsbBroadEn_03728
- DNA Barcode:
- TGTAAAGCACTTATAACTACAAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DIABLO (56616)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474122
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56616 | DIABLO | diablo IAP-binding mitochon... | NM_001371333.1 | 100% | 100% | |
| 2 | human | 56616 | DIABLO | diablo IAP-binding mitochon... | NM_019887.6 | 100% | 100% | |
| 3 | human | 56616 | DIABLO | diablo IAP-binding mitochon... | NM_001278342.1 | 81.5% | 81.5% | 181_182ins132 |
| 4 | human | 56616 | DIABLO | diablo IAP-binding mitochon... | NM_001278304.2 | 77.1% | 74.8% | (many diffs) |
| 5 | human | 56616 | DIABLO | diablo IAP-binding mitochon... | NM_138930.3 | 77.1% | 74.8% | (many diffs) |
| 6 | human | 56616 | DIABLO | diablo IAP-binding mitochon... | NM_001278303.1 | 69.4% | 69.4% | 0_1ins219 |
| 7 | human | 56616 | DIABLO | diablo IAP-binding mitochon... | NM_001278302.1 | 58.1% | 56.4% | (many diffs) |
| 8 | mouse | 66593 | Diablo | diablo, IAP-binding mitocho... | NM_023232.3 | 84.1% | 84.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 783
- ORF length:
- 717
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggctctgaag agttggctgt cgcgcagcgt aacttcattc ttcaggtaca 121 gacagtgttt gtgtgttcct gttgtggcta actttaagaa gcggtgtttc tcagaattga 181 taagaccatg gcacaaaact gtgacgattg gctttggagt aaccctgtgt gcggttccta 241 ttgcacagaa atcagagcct cattccctta gtagtgaagc attgatgagg agagcagtgt 301 ctttggTAAC AGATAGCACC TCTACCTTTC TCTCTCAGAC CACATATGCG TTGATTGAAG 361 CTATTACTGA ATATACTAAG GCTGTTTATA CCTTAACTTC TCTTTACCGA CAATATACAA 421 GTTTACTTGG GAAAATGAAT TCAGAGGAGG AAGATGAAGT GTGGCAGGTG ATCATAGGAG 481 CCAGAGCTGA GATGACTTCA AAACACCAAG AGTACTTGAA GCTGGAAACC ACTTGGATGA 541 CTGCAGTTGG TCTTTCAGAG ATGGCAGCAG AAGCTGCATA TCAAACTGGC GCAGATCAGG 601 CCTCTATAAC CGCCAGGAAT CACATTCAGC TGGTGAAACT GCAGGTGGAA GAGGTGCACC 661 AGCTCTCCCG GAAAGCAGAA ACCAAGCTGG CAGAAGCACA GATAGAAGAG CTCCGTCAGA 721 AAACACAGGA GGAAGGGGAG GAGCGGGCTG AGTCGGAGCA GGAGGCCTAC CTGCGTGAGG 781 ATTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 841 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 901 GGCTTTATAT ATCTTGTGGA AAGGACGATG TAAAGCACTT ATAACTACAA GCACGCGTTA 961 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt