Construct: ORF TRCN0000474125

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015611.1_s317c1
Derived from:
ccsbBroadEn_04070
DNA Barcode:
GACTGACCGACTGGTCCCCTAGCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TMEM43 (79188)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474125
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79188 TMEM43 transmembrane protein 43 NM_024334.2 100% 100%
2 human 79188 TMEM43 transmembrane protein 43 XM_017007176.2 91.2% 91.2% 0_1ins105
3 mouse 74122 Tmem43 transmembrane protein 43 NM_028766.3 86.6% 93% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1266
ORF length:
1200
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc cgcgaattat tccagtacca gtacccggag agaacatgtc aaagttaaaa
121ccagctccca gccaggcttc ctggaacggc tgagcgagac ctcgggtggg atgtttgtgg
181ggctcatggc cttcctgctc tccttctacc taattttcac caatgagggc cgcgcattga
241agacggcaac ctcattggct gaggggctct cgcttgtggt gtctcccgac agcatccaca
301gtgtggctcc ggagaatgaa ggaaggctgg tgcacatcat tggcgcctta cggacatcca
361agcttttgtc tgatccaaac tatggggtcc atcttccggc tgtgaaactg cggaggcacg
421tggagatgta ccaatgggta gaaactgagg agtccaggga gtacaccgag gatgggcagg
481tgaagaagga gacgaggtat tcctacaaca ctgaatggag gtcagaaatc atcaacagca
541aaaacttcga ccgagagatt ggccacaaaa accccagtgc catggcagtg gagtcattca
601tggcaacagc cccctttgtc caaattggca ggtttttcct ctcgtcaggc ctcatcgaca
661aagtcgacaa cttcaagtcc ctgagcctat ccaagctgga ggaccctcat gtggacatca
721ttcgccgtgg agactttttc taccacagcg aaaatcccaa gtatccagag gtgggagact
781tgcgtgtctc cttttcctat gctggactga gcggcgatga ccctgacctg ggcccagctc
841acgtggtcac tgtgattgcc cggcagcggg gtgaccagct agtcccattc tccaccaagt
901ctggggatac cttactgctc cTGCACCACG GGGACTTCTC AGCAGAGGAG GTGTTTCATA
961GAGAACTAAG GAGCAACTCC ATGAAGACCT GGGGCCTGCG GGCAGCTGGC TGGATGGCCA
1021TGTTCATGGG CCTCAACCTT ATGACACGGA TCCTCTACAC CTTGGTGGAC TGGTTTCCTG
1081TTTTCCGAGA CCTGGTCAAC ATTGGCCTGA AAGCCTTTGC CTTCTGTGTG GCCACCTCGC
1141TGACCCTGCT GACCGTGGCG GCTGGCTGGC TCTTCTACCG ACCCCTGTGG GCCCTCCTCA
1201TTGCCGGCCT GGCCCTTGTG CCCATCCTTG TTGCTCGGAC ACGGGTGCCA GCCAAAAAGT
1261TGGAGTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
1321TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
1381CTTGGCTTTA TATATCTTGT GGAAAGGACG AGACTGACCG ACTGGTCCCC TAGCCACGCG
1441TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)