Construct: ORF TRCN0000474133
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010836.1_s317c1
- Derived from:
- ccsbBroadEn_02186
- DNA Barcode:
- GTGCCCCCTTAACCACTTTGTTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BAG2 (9532)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474133
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9532 | BAG2 | BAG cochaperone 2 | NM_004282.4 | 100% | 100% | |
| 2 | human | 9532 | BAG2 | BAG cochaperone 2 | XM_005249490.4 | 82.6% | 81.9% | (many diffs) |
| 3 | human | 9532 | BAG2 | BAG cochaperone 2 | XM_011514998.2 | 82.6% | 81.9% | (many diffs) |
| 4 | human | 9532 | BAG2 | BAG cochaperone 2 | XM_011514999.3 | 82.6% | 81.9% | (many diffs) |
| 5 | human | 9532 | BAG2 | BAG cochaperone 2 | XM_024446593.1 | 82.6% | 81.9% | (many diffs) |
| 6 | human | 9532 | BAG2 | BAG cochaperone 2 | XM_024446594.1 | 82.6% | 81.9% | (many diffs) |
| 7 | human | 9532 | BAG2 | BAG cochaperone 2 | XM_024446595.1 | 82.6% | 81.9% | (many diffs) |
| 8 | human | 9532 | BAG2 | BAG cochaperone 2 | XM_024446596.1 | 82.6% | 81.9% | (many diffs) |
| 9 | mouse | 213539 | Bag2 | BCL2-associated athanogene 2 | NM_145392.2 | 85% | 93.3% | (many diffs) |
| 10 | mouse | 213539 | Bag2 | BCL2-associated athanogene 2 | XM_017319691.1 | 60% | 67.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 702
- ORF length:
- 633
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctcaggcg aagatcaacg ctaaagccaa cgaggggcgc ttctgccgct 121 cctcctccat ggctgaccgc tccagccgcc tgctggagag cctggaccag ctggagctca 181 gggttgaagc tttgagagaa gcagcaactg ctgttgagca agagaaagaa atccTTCTGG 241 AAATGATCCA CAGTATCCAA AATAGCCAGG ACATGAGGCA GATCAGTGAC GGAGAAAGAG 301 AAGAATTAAA TCTGACTGCA AACCGTTTGA TGGGAAGAAC TCTCACCGTT GAAGTGTCAG 361 TAGAAACAAT TAGAAACCCC CAGCAGCAAG AATCCCTAAA GCATGCCACA AGGATTATTG 421 ATGAGGTGGT CAATAAGTTT CTGGATGATT TGGGAAATGC CAAGAGTCAT TTAATGTCGC 481 TCTACAGTGC ATGTTCATCT GAGGTGCCAC ATGGGCCAGT TGATCAGAAG TTTCAATCCA 541 TAGTAATTGG CTGTGCTCTT GAAGATCAGA AGAAAATTAA GAGAAGATTA GAGACTCTGC 601 TTAGAAATAT TGAAAACTCT GACAAGGCCA TCAAGCTATT AGAGCATTCT AAAGGAGCTG 661 GTTCCAAAAC TCTGCAACAA AATGCTGAAA GCAGATTCAA TTTGCCAACT TTCTTGTACA 721 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 781 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 841 AGGACGAGTG CCCCCTTAAC CACTTTGTTT TACGCGTTAA GTCgacaatc aacctctgga 901 ttacaaaatt tgtgaaagat t