Construct: ORF TRCN0000474134
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000783.1_s317c1
- Derived from:
- ccsbBroadEn_08753
- DNA Barcode:
- AATCGAGTCAGAACACCTCGGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POGK (57645)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474134
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57645 | POGK | pogo transposable element d... | NM_017542.5 | 99.9% | 99.8% | 1827C>A |
| 2 | human | 57645 | POGK | pogo transposable element d... | XM_005245370.4 | 99.9% | 99.8% | 1827C>A |
| 3 | human | 57645 | POGK | pogo transposable element d... | XM_011509814.2 | 99.9% | 99.8% | 1827C>A |
| 4 | human | 57645 | POGK | pogo transposable element d... | XM_017001914.2 | 99.9% | 99.8% | 1827C>A |
| 5 | human | 57645 | POGK | pogo transposable element d... | XM_011509815.2 | 96.9% | 96.8% | 1154_1155ins54;1773C>A |
| 6 | human | 57645 | POGK | pogo transposable element d... | NM_001314014.2 | 80.5% | 80.2% | 1_1delAins353;3_4insCA;1473C>A |
| 7 | human | 57645 | POGK | pogo transposable element d... | XM_017001915.1 | 80.5% | 80.2% | 1_1delAins353;3_4insCA;1473C>A |
| 8 | mouse | 71592 | Pogk | pogo transposable element w... | NM_175170.4 | 85.9% | 89.9% | (many diffs) |
| 9 | mouse | 71592 | Pogk | pogo transposable element w... | NM_001142948.1 | 84.3% | 88.2% | (many diffs) |
| 10 | mouse | 71592 | Pogk | pogo transposable element w... | XM_017312357.1 | 79.9% | 84.2% | (many diffs) |
| 11 | mouse | 71592 | Pogk | pogo transposable element w... | XM_011238869.2 | 70.3% | 74.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1896
- ORF length:
- 1827
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagtccaca gcctaccctc tcaatttgag cctgaaagaa gaggaagagg 121 aagaagagat tcagagccgg gaactagagg acggcccggc agacatgcag aaagtacgaa 181 tctgctctga gggcggatgg gtaccggccc tatttgatga ggtggccata tatttttccg 241 atgaggaatg ggaagttttg acggagcaac aaaaggccct ctaccgggaa gtcatgagga 301 tgaattatga aactgtcctg tccctggaat tcccattccc taagccagac atgatcaccc 361 gtttggaagg ggaggaggag tctcagaatt ctgacgagtg gcagctccaa ggaggcacct 421 ctgcagaaaa tgaagaatct gacgtaaagc ctccagactg gccaaaccca atgaatgcta 481 cctcccagtt tcctcagcct cagcactttg acagctttgg cctccgtctg cctcgggata 541 tcacagagct gcccgagtgg agtgaggggt accccttcta catggccatg ggcttcccag 601 ggtatgacct ctcggctgat gacatagctg ggaagtttca gttcagccgg ggcatgcgcc 661 gcagttacga cgcagggttc aagctgatgg tagtggaata tgctgagagt accaacaact 721 gccaggctgc caagcagttt ggagtattgg aaaaaaacgt tcgagactgg cgcaaagtga 781 agccacagct tcaaaacgcc cacgccatgc ggcgggcatt ccgaggcccc aagaatggga 841 ggtttgctct ggtggaccag cgtgtggccg aatatgtcag atacatgcag gccaaagggg 901 accccatcac ccgggaggcg atgcagctga aagctctcga aatcgcccag gaaatgaaca 961 ttccagagaa agggttcaag gcaagcttgg gttggtgtcg aagaatgatg agaaggtatg 1021 acctgtctct gaggcataaa gtgcccgtgc cccagcacct gccggaagac ctgactgaga 1081 aactcgtcac ttaccagcgc agtgtcctgg ctctgcgcag ggcgcatgac tatgaggtag 1141 ctcagatggg gaatgcagat gagacgccca tttgtttaga ggtgccatca cgggtaactg 1201 ttgataacca gggcgaaaag cctgtcttgg tcaagacacc aggcagggaa aaactgaaaa 1261 tcacagcaat gcttggtgtc ttggctgatg ggaggaagtt accaccgtac atcattttga 1321 ggggaacata tatccccccg gggaagtttc ccagtgggat ggaaattcgc tgccaccggt 1381 atgggtggat gactgaagac ttGATGCAGG ACTGGTTGGA AGTGGTGTGG AGACGGAGGA 1441 CAGGAGCAGT GCCCAAGCAG CGAGGGATGC TGATCTTGAA TGGCTTCCGG GGCCATGCCA 1501 CAGATTCCGT GAAGAACTCC ATGGAAAGCA TGAACACTGA CATGGTGATC ATCCCAGGGG 1561 GTCTGACCTC ACAGCTTCAG GTGCTGGATG TCGTGGTCTA CAAGCCACTG AATGACAGTG 1621 TGCGGGCCCA GTACTCCAAC TGGCTTCTGG CTGGGAACCT GGCGCTGAGC CCAACCGGGA 1681 ATGCTAAGAA GCCACCCCTG GGCCTCTTTC TGGAGTGGGT CATGGTCGCG TGGAATAGCA 1741 TCTCAAGTGA GTCCATCGTC CAAGGGTTCA AGAAGTGCCA TATCTCCAGC AACTTGGAGG 1801 AGGAAGACGA TGTCCTGTGG GAAATCGAGA GTGAGTTGCC AGGAGGAGGA GAACCACCAA 1861 AAGATTGTGA CACCGAAAGC ATGGCTGAGA GCAAATTGCC AACTTTCTTG TACAAAGTGG 1921 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1981 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2041 AAATCGAGTC AGAACACCTC GGCGCACGCG TTAAGTCgac aatcaacctc tggattacaa 2101 aatttgtgaa agatt