Construct: ORF TRCN0000474142
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004759.1_s317c1
- Derived from:
- ccsbBroadEn_14092
- DNA Barcode:
- ACATGGTAAGTTCTGCATGAAACC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SSBP3 (23648)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474142
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23648 | SSBP3 | single stranded DNA binding... | XM_017000899.2 | 81.1% | 1.5% | 1_141del;148delC |
| 2 | human | 23648 | SSBP3 | single stranded DNA binding... | XM_017000897.2 | 73.2% | 1.4% | 1_222del;229delC |
| 3 | human | 23648 | SSBP3 | single stranded DNA binding... | XM_017000898.2 | 73.2% | 1.4% | 1_222del;229delC |
| 4 | human | 23648 | SSBP3 | single stranded DNA binding... | XM_006710545.4 | 59.7% | 1.1% | 1_411del;418delC |
| 5 | human | 23648 | SSBP3 | single stranded DNA binding... | NM_001009955.4 | 56.4% | 1.1% | 1_471del;478delC |
| 6 | human | 23648 | SSBP3 | single stranded DNA binding... | NM_018070.5 | 55.3% | 1% | 1_492del;499delC |
| 7 | human | 23648 | SSBP3 | single stranded DNA binding... | NM_145716.4 | 52.4% | 1% | 1_552del;559delC |
| 8 | mouse | 72475 | Ssbp3 | single-stranded DNA binding... | XM_006503420.2 | 69.9% | 1.4% | (many diffs) |
| 9 | mouse | 72475 | Ssbp3 | single-stranded DNA binding... | XM_006503419.2 | 68.7% | 1.4% | (many diffs) |
| 10 | mouse | 72475 | Ssbp3 | single-stranded DNA binding... | XM_006503417.3 | 63.6% | 1.3% | (many diffs) |
| 11 | mouse | 72475 | Ssbp3 | single-stranded DNA binding... | NM_198438.1 | 52.9% | 1.1% | (many diffs) |
| 12 | mouse | 72475 | Ssbp3 | single-stranded DNA binding... | XM_006503416.2 | 51.7% | 1% | (many diffs) |
| 13 | mouse | 72475 | Ssbp3 | single-stranded DNA binding... | XM_006503415.2 | 50.9% | 1% | (many diffs) |
| 14 | mouse | 72475 | Ssbp3 | single-stranded DNA binding... | XM_006503414.2 | 49.8% | 1% | (many diffs) |
| 15 | mouse | 72475 | Ssbp3 | single-stranded DNA binding... | NM_023672.2 | 49.2% | 1% | (many diffs) |
| 16 | mouse | 72475 | Ssbp3 | single-stranded DNA binding... | XM_006503413.2 | 48.2% | 1% | (many diffs) |
| 17 | mouse | 72475 | Ssbp3 | single-stranded DNA binding... | XM_006503412.2 | 47.5% | .9% | (many diffs) |
| 18 | mouse | 72475 | Ssbp3 | single-stranded DNA binding... | XM_006503411.2 | 46.5% | .9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 120
- ORF length:
- 54
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tccacacgac aacaaggcca ccccaacatg ggaggatcaa tgcagagaat 121 gaaccctccc cgaggcatgg ggcccatggg tcccggccca cagaattacg gcagcggcat 181 gagaccacca cccaactccc TCGGCCCCGC CATGCCCGGG ATTAACATGG GCCCGGGAGC 241 TGGCAGACCC TGGCCCAATC CTAACAGTGC TAACTCAATT CCATACTCCT CCTCATCACC 301 TGGTACCTAT GTGGGACCCC CTGGTGGTGG CGGTCCTCCA GGAACACCCA TTATGCCCAG 361 TCCCGCAGAT TCAACAAATT CCAGTGACAA CATCTACACA ATGATTAATC CAGTGCCGCC 421 TGGAGGCAGC CGGTCCAACT TCCCGATGGG TCCCGGCTCG GACGGTCCGA TGGGCGGCAT 481 GGGTGGCATG GAGCCACACC ACATGAATGG ATCATTAGGG TCAGGCGACA TAGACGGACT 541 TCCAAAAAAT TCTCCTAACA ACATAAGTGG CATTAGCAAT CCTCCAGGCA CCCCTCGAGA 601 TGACGGCGAG CTAGGAGGGA ACTTCCTCCA CTCCTTTCAG AACGACAATT ATTCTCCAAG 661 CATGACGATG AGTGTGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT 721 CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG 781 TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACATGGTA AGTTCTGCAT 841 GAAACCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt