Construct: ORF TRCN0000474144
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010655.1_s317c1
- Derived from:
- ccsbBroadEn_09166
- DNA Barcode:
- ACGATATGGAATGTTGGTTCGGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HSDL2 (84263)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474144
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84263 | HSDL2 | hydroxysteroid dehydrogenas... | NM_001195822.1 | 99.9% | 100% | 1032A>G |
| 2 | human | 84263 | HSDL2 | hydroxysteroid dehydrogenas... | NM_032303.5 | 82.4% | 82.5% | 280_498del;1251A>G |
| 3 | human | 84263 | HSDL2 | hydroxysteroid dehydrogenas... | XM_017015203.2 | 70.6% | 70.7% | 0_1ins303;729A>G |
| 4 | human | 84263 | HSDL2 | hydroxysteroid dehydrogenas... | XM_017015204.2 | 70.6% | 70.7% | 0_1ins303;729A>G |
| 5 | human | 84263 | HSDL2 | hydroxysteroid dehydrogenas... | XM_017015205.2 | 70.6% | 70.7% | 0_1ins303;729A>G |
| 6 | human | 84263 | HSDL2 | hydroxysteroid dehydrogenas... | XM_011519091.3 | 49.6% | 46.5% | (many diffs) |
| 7 | human | 84263 | HSDL2 | hydroxysteroid dehydrogenas... | NR_036651.1 | 29.4% | (many diffs) | |
| 8 | mouse | 72479 | Hsdl2 | hydroxysteroid dehydrogenas... | XM_006538277.2 | 64.7% | 61.9% | (many diffs) |
| 9 | mouse | 72479 | Hsdl2 | hydroxysteroid dehydrogenas... | NM_024255.3 | 61.5% | 61.2% | (many diffs) |
| 10 | mouse | 72479 | Hsdl2 | hydroxysteroid dehydrogenas... | XM_017320391.1 | 57.4% | 55.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1101
- ORF length:
- 1035
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt acccaacacc gggaggctgg caggatgtac agtttttatc acaggtgcaa 121 gccgtggcat tggcaaagct attgcattga aagcagcaaa ggatggagca aatattgtta 181 ttgctgcaaa gaccgcccag ccacatccaa aacttctagg cacaatctat actgctgctg 241 aagaaattga agcagttgga ggaaaggcct tgccatgtat tgttgatgtg agagatgaac 301 agcagatcag tgctgcagtg gagaaagcca tcaagaaatt tggagcttat accattgcta 361 agtatggtat gtctatgtat gtgcttggaa tggcagaaga atttaaaggt gaaattgcag 421 tcaatgcatt atggcctaaa acagccatac acactgctgc tatggatatg ctgggaggac 481 ctggtatcga aagccagtgt agaaaagttg atatcattgc agatgcagca TATTCCATTT 541 TCCAAAAGCC AAAAAGTTTT ACTGGCAACT TTGTCATTGA TGAAAATATC TTAAAAGAAG 601 AAGGAATAGA AAATTTTGAC GTTTATGCAA TTAAACCAGG TCATCCTTTG CAACCAGATT 661 TCTTCTTAGA TGAATACCCA GAAGCAGTTA GCAAGAAAGT GGAATCAACT GGTGCTGTTC 721 CAGAATTCAA AGAAGAGAAA CTGCAGCTGC AACCAAAACC ACGTTCTGGA GCTGTGGAAG 781 AAACATTTAG AATTGTTAAG GACTCTCTCA GTGATGATGT TGTTAAAGCC ACTCAAGCAA 841 TCTATCTGTT TGAACTCTCC GGTGAAGATG GTGGCACGTG GTTTCTTGAT CTGAAAAGCA 901 AGGGTGGGAA TGTCGGATAT GGAGAGCCTT CTGATCAGGC AGATGTGGTG ATGAGTATGA 961 CTACTGATGA CTTTGTAAAA ATGTTTTCAG GGAAACTAAA ACCAACAATG GCATTCATGT 1021 CAGGGAAATT GAAGATTAAA GGTAACATGG CCCTAGCAAT CAAATTGGAG AAGCTAATGA 1081 ATCAGATGAA TGCCAGGCTG TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1141 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1201 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACGA TATGGAATGT 1261 TGGTTCGGTC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt