Transcript: Human NM_032303.5

Homo sapiens hydroxysteroid dehydrogenase like 2 (HSDL2), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-28
Taxon:
Homo sapiens (human)
Gene:
HSDL2 (84263)
Length:
3174
CDS:
57..1313

Additional Resources:

NCBI RefSeq record:
NM_032303.5
NBCI Gene record:
HSDL2 (84263)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_032303.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000064934 GCTTATACCATTGCTAAGTAT pLKO.1 555 CDS 100% 5.625 7.875 N HSDL2 n/a
2 TRCN0000300135 GCTTATACCATTGCTAAGTAT pLKO_005 555 CDS 100% 5.625 7.875 N HSDL2 n/a
3 TRCN0000064935 CGTGGCATTGGCAAAGCTATT pLKO.1 114 CDS 100% 10.800 7.560 N HSDL2 n/a
4 TRCN0000300195 CGTGGCATTGGCAAAGCTATT pLKO_005 114 CDS 100% 10.800 7.560 N HSDL2 n/a
5 TRCN0000064937 GTGCCATTAGTTTGACCAATA pLKO.1 364 CDS 100% 10.800 7.560 N HSDL2 n/a
6 TRCN0000300158 GTGCCATTAGTTTGACCAATA pLKO_005 364 CDS 100% 10.800 7.560 N HSDL2 n/a
7 TRCN0000064936 CCAGAAGCAGTTAGCAAGAAA pLKO.1 888 CDS 100% 5.625 3.938 N HSDL2 n/a
8 TRCN0000300196 CCAGAAGCAGTTAGCAAGAAA pLKO_005 888 CDS 100% 5.625 3.938 N HSDL2 n/a
9 TRCN0000064933 CCTAGCAATCAAATTGGAGAA pLKO.1 1262 CDS 100% 4.050 2.835 N HSDL2 n/a
10 TRCN0000099636 GCAATCAAATTGGAGAAGCTA pLKO.1 1266 CDS 100% 3.000 2.100 N Hsdl2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_032303.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09166 pDONR223 100% 82.4% 82.5% None 280_498del;1251A>G n/a
2 ccsbBroad304_09166 pLX_304 0% 82.4% 82.5% V5 280_498del;1251A>G n/a
3 TRCN0000474144 ACGATATGGAATGTTGGTTCGGTC pLX_317 55% 82.4% 82.5% V5 280_498del;1251A>G n/a
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