Construct: ORF TRCN0000474157
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014191.1_s317c1
- Derived from:
- ccsbBroadEn_09348
- DNA Barcode:
- GGATTAAATGATAGTTATTCTTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM114A1 (92689)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474157
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92689 | FAM114A1 | family with sequence simila... | NM_138389.4 | 99.9% | 100% | 1497T>C |
| 2 | human | 92689 | FAM114A1 | family with sequence simila... | XM_005262672.2 | 99.9% | 100% | 1497T>C |
| 3 | human | 92689 | FAM114A1 | family with sequence simila... | NM_001350632.2 | 99.5% | 99.4% | 550_551insTAACAG;1491T>C |
| 4 | human | 92689 | FAM114A1 | family with sequence simila... | NM_001350633.2 | 77.3% | 74.6% | 0_1ins377;55_56insGTAG;1116T>C |
| 5 | human | 92689 | FAM114A1 | family with sequence simila... | NM_001350635.2 | 74.3% | 68% | (many diffs) |
| 6 | human | 92689 | FAM114A1 | family with sequence simila... | NM_001350634.2 | 64.7% | 60.2% | 0_1ins556;63_64ins38;903T>C |
| 7 | human | 92689 | FAM114A1 | family with sequence simila... | NM_001330764.2 | 63.1% | 63.2% | 0_1ins621;876T>C |
| 8 | human | 92689 | FAM114A1 | family with sequence simila... | NM_001350631.2 | 56.1% | 54.6% | (many diffs) |
| 9 | human | 92689 | FAM114A1 | family with sequence simila... | NR_033290.2 | 35.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1755
- ORF length:
- 1689
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgatgatgct ggtgacacct tagccactgg agacaaagca gaagttactg 121 agatgcctaa tagtgattct ttacctgagg atgcagaagt gcattgtgat tcagctgcag 181 tttcacatga gccaacacca gctgacccca gaggggaggg gcatgaaaat gcagctgtgc 241 agggtgcagg ggctgccgcc attgggcccc ctgtgcagcc tcaggatgcc aacgccctgg 301 agccccctct caatggagac gtgactgagg atacacttgc tgaatgtatt gattccgtca 361 gccttgaggc agaacccaga tccgaaatac ccctgcaaga acagaattat ctggctgtgg 421 attcccctcc aagtggagga ggatgggcag gctggggatc ctggggcaaa tctctgctgt 481 cgtcagcatc tgccacagta ggtcatggat tgacggcagt caaggaaaaa gcaggagcca 541 ctctacggat tcatggtgta aattctggat cttctgaagg agcccaacca aatactgaaa 601 acggagtccc tgaaataaca gatgcagcca cagatcaggg ccctgcagaa agcccaccca 661 cttccccttc atcagcctct cggggtatgc tgtctgccat caccaatgtg gttcaaaaca 721 caggtaaaag tgtcttaact ggaggccttg atgcgttgga attcatcggc aagaaaacca 781 tgaatgtcct tgcagaaagt gacccgggct ttaagcggac caagacgctc atggagagaa 841 ctgtttcctt gtctcagatg ttaagggaag ctaaggagaa ggagaagcag agactggcac 901 agcagctcac gatggagaga accgcgcact acgggatgct gtttgatgaa tatcaaggct 961 tgtcacacct ggaagccctg gaaattctgt ccaatgaaag cgaaagcaag gttcagtcat 1021 ttttagcatc acttgatgga gagaagctgg aactcttaaa aaatgaccta atttccatta 1081 aagacatctt tgcagccaaa gaattagaga atgaagaaaa tcaagaagaa caaggcttag 1141 aagaaaaggg agaagaattt gctcgcatgc ttacagagct tctctttgaa ttacatgtgg 1201 cggccacacc tgacaaactc aataaggcca tgaagagggc tcatgactgg gtGGAAGAGG 1261 ATCAAACCGT GGTGTCAGTA GATGTGGCAA AAGTGTCCGA AGAAGAAACA AAGAAGGAAG 1321 AAAAGGAAGA GAAATCTCAA GACCCTCAAG AAGACAAAAA GGAGGAAAAG AAAACTAAGA 1381 CCATAGAGGA AGTATACATG TCGTCCATTG AAAGTCTGGC GGAGGTAACA GCGCGCTGTA 1441 TTGAGCAGCT TCATAAAGTA GCAGAATTAA TTCTTCATGG ACAAGAAGAG GAAAAACCAG 1501 CTCAGGACCA AGCAAAAGTT CTAATAAAAT TAACTACTGC AATGTGCAAT GAAGTGGCCT 1561 CCTTATCAAA GAAGTTTACG AATTCTTTAA CCACTGTTGG GAGCAACAAG AAGGCCGAGG 1621 TCCTTAACCC CATGATCAGT AGTGTATTGT TAGAGGGCTG CAACAGTACA ACGTACATAC 1681 AGGATGCCTT CCAGCTGCTG CTGCCTGTTC TGCAGGTCTC ACATATCCAG ACCAGTTGTT 1741 TGAAAGCACA GCCGTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1801 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1861 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GGATTAAATG ATAGTTATTC 1921 TTACACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt