Construct: ORF TRCN0000474168
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005919.1_s317c1
- Derived from:
- ccsbBroadEn_11282
- DNA Barcode:
- AATTTGACGACATGACATTACAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RGS20 (8601)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474168
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8601 | RGS20 | regulator of G protein sign... | NM_003702.4 | 90% | 90% | 1_72del |
| 2 | human | 8601 | RGS20 | regulator of G protein sign... | NM_001286673.2 | 79.4% | 79.4% | 1_168del |
| 3 | human | 8601 | RGS20 | regulator of G protein sign... | NM_001286675.2 | 79.4% | 78.8% | (many diffs) |
| 4 | human | 8601 | RGS20 | regulator of G protein sign... | NM_001286674.2 | 68.6% | 63.1% | (many diffs) |
| 5 | human | 8601 | RGS20 | regulator of G protein sign... | NM_170587.4 | 55.9% | 55.9% | 1_513del |
| 6 | human | 8601 | RGS20 | regulator of G protein sign... | NR_104579.1 | 37.6% | (many diffs) | |
| 7 | mouse | 58175 | Rgs20 | regulator of G-protein sign... | XM_006495554.2 | 58.1% | 59.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 717
- ORF length:
- 651
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg atcagagcgg atggagatgc ggaagcggca gatgcccgcc gcccaggaca 121 caccaggcgc cgccccaggc cagcccggag cggggagtcg cgggtccaac gcatgctgct 181 tctgctggtg ctgctgttGT AGCTGCTCGT GTCTCACTGT TAGAAACCAG GAAGATCAGA 241 GGCCCACAAT AGCTTCCCAC GAACTCAGAG CAGATCTTCC AACCTGGGAA GAAAGCCCTG 301 CTCCTACTCT GGAAGAAGTC AACGCCTGGG CTCAGTCATT TGACAAATTA ATGGTCACTC 361 CAGCAGGAAG GAATGCATTC CGTGAATTCC TCCGAACAGA ATTCAGTGAG GAAAATATGC 421 TCTTCTGGAT GGCCTGTGAG GAACTGAAAA AGGAAGCTAA TAAAAACATT ATTGAAGAGA 481 AAGCAAGGAT AATCTATGAA GACTACATTT CTATACTTTC TCCTAAGGAG GTGAGCTTAG 541 ACTCCCGGGT GAGAGAAGTG ATCAACAGAA ACATGGTGGA GCCATCCCAA CACATATTCG 601 ATGATGCTCA ACTTCAGATT TACACCCTGA TGCACAGAGA CTCATATCCT CGATTCATGA 661 ACTCTGCTGT CTATAAGGAC TTGCTTCAGT CCTTATCGGA GAAATCTATT GAAGCATACC 721 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 781 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 841 ATATATCTTG TGGAAAGGAC GAAATTTGAC GACATGACAT TACAACACGC GTTAAGTCga 901 caatcaacct ctggattaca aaatttgtga aagatt