Construct: ORF TRCN0000474179
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001627.1_s317c1
- Derived from:
- ccsbBroadEn_00469
- DNA Barcode:
- GGTTCTGTGACTTCCATAACCATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DUT (1854)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474179
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1854 | DUT | deoxyuridine triphosphatase | NM_001948.4 | 100% | 100% | |
| 2 | human | 1854 | DUT | deoxyuridine triphosphatase | NM_001330286.2 | 96% | 95.2% | (many diffs) |
| 3 | human | 1854 | DUT | deoxyuridine triphosphatase | NM_001025249.1 | 85.9% | 85.9% | 0_1ins69 |
| 4 | human | 1854 | DUT | deoxyuridine triphosphatase | NM_001025248.2 | 64.2% | 63.4% | (many diffs) |
| 5 | human | 1854 | DUT | deoxyuridine triphosphatase | XM_017021988.1 | 41% | 38.3% | (many diffs) |
| 6 | human | 1854 | DUT | deoxyuridine triphosphatase | XR_931760.1 | 35.2% | (many diffs) | |
| 7 | human | 1854 | DUT | deoxyuridine triphosphatase | XR_001751125.2 | 21.8% | (many diffs) | |
| 8 | mouse | 110074 | Dut | deoxyuridine triphosphatase | NM_023595.6 | 81.5% | 86.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 558
- ORF length:
- 492
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ctgctctgaa gagacacccg ccatttcacc cagtaagcgg gcccggcctG 121 CGGAGGTGGG CGGCATGCAG CTCCGCTTTG CCCGGCTCTC CGAGCACGCC ACGGCCCCCA 181 CCCGGGGCTC CGCGCGCGCC GCGGGCTACG ACCTGTACAG TGCCTATGAT TACACAATAC 241 CACCTATGGA GAAAGCTGTT GTGAAAACGG ACATTCAGAT AGCGCTCCCT TCTGGGTGTT 301 ATGGAAGAGT GGCTCCACGG TCAGGCTTGG CTGCAAAACA CTTTATTGAT GTAGGAGCTG 361 GTGTCATAGA TGAAGATTAT AGAGGAAATG TTGGTGTTGT ACTGTTTAAT TTTGGCAAAG 421 AAAAGTTTGA AGTCAAAAAA GGTGATCGAA TTGCACAGCT CATTTGCGAA CGGATTTTTT 481 ATCCAGAAAT AGAAGAAGTT CAAGCCTTGG ATGACACCGA AAGGGGTTCA GGAGGTTTTG 541 GTTCCACTGG AAAGAATTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 601 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGCCCG TAACTTGAAA 661 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGGTTCTG TGACTTCCAT 721 AACCATGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt