Construct: ORF TRCN0000474180
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011334.1_s317c1
- Derived from:
- ccsbBroadEn_11070
- DNA Barcode:
- CTCCAAGACGGCAAAGGCGTAGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTH1R (5745)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474180
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | XM_017006934.1 | 64.5% | 63.9% | 175_213del;1026_1086del;1100_1548del |
| 2 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | NM_000316.3 | 56.1% | 55.6% | 987_1047del;1061_1779del |
| 3 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | NM_001184744.1 | 56.1% | 55.6% | 987_1047del;1061_1779del |
| 4 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | XM_017006933.1 | 56.1% | 55.6% | 987_1047del;1061_1779del |
| 5 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | XM_011533967.3 | 54.9% | 54.4% | 175_213del;1026_1086del;1100_1818del |
| 6 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | XM_017006932.2 | 54.9% | 54.4% | 175_213del;1026_1086del;1100_1818del |
| 7 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | XM_011533968.2 | 52.4% | 49.8% | (many diffs) |
| 8 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | XM_005265344.3 | 50.9% | 50.4% | 0_1ins93;894_954del;968_1686del |
| 9 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | NM_001083935.1 | 48.9% | 50.4% | (many diffs) |
| 10 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | NM_001083936.1 | 48.9% | 50.4% | (many diffs) |
| 11 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | NM_011199.2 | 48.9% | 50.4% | (many diffs) |
| 12 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | XM_011242942.1 | 47.1% | 45.3% | (many diffs) |
| 13 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | XM_006511988.3 | 46.8% | 46.5% | (many diffs) |
| 14 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | XM_017313215.1 | 46.8% | 46.5% | (many diffs) |
| 15 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | XM_017313214.1 | 44.5% | 42.9% | (many diffs) |
| 16 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | XM_006511985.2 | 39.4% | 38% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1065
- ORF length:
- 999
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gaccgcccgg atcgcacccg gcctggcgct cctgctctgc tgccccgtgc 121 tcagctccgc gtacgcgctg gtggatgcag atgacgtcat gactaaagag gaacagatct 181 tcctgctgca ccgtgctcag gcccagtgcg aaaaacggct caaggaggtc ctgcagaggc 241 cagccagcat aatggaatca gacaagggat ggacatctgc gtccacatca gggaagccca 301 ggaaagataa ggcatctggg aagctctacc ctgagtctga ggaggacaag gaggcaccca 361 ctggcagcag gtaccgaggg cgcccctgtc tgccggaatg ggaccacatc ctgtgctggc 421 cgctgggggc accaggtgag gtggtggctg tgccctgtcc ggactacatt tatgacttca 481 atcacaaagg ccatgcctac cgacgctgtg accgcaatgg cagctgggag ctggtgcctg 541 ggcacaacag gacgtgggcc aactacagcg agtgtgtcaa aTTTCTCACC AATGAGACTC 601 GTGAACGGGA GGTGTTTGAC CGCCTGGGCA TGATTTACAC CGTGGGCTAC TCCGTGTCCC 661 TGGCGTCCCT CACCGTAGCT GTGCTCATCC TGGCCTACTT TAGGCGGCTG CACTGCACGC 721 GCAACTACAT CCACATGCAC CTGTTCCTGT CCTTCATGCT GCGCGCCGTG AGCATCTTCG 781 TCAAGGACGC TGTGCTCTAC TCTGGCGCCA CGCTTGATGA GGCTGAGCGC CTCACCGAGG 841 AGGAGCTGCG CGCCATCGCC CAGGCGCCCC CGCCGCCTGC CACCGCCGCT GCCGGCTACG 901 CGGGCTGCAG GGTGGCTGTG ACCTTCTTCC TTTACTTCCT GGCCACCAAC TACTACTGGA 961 TTCTGGTGGA GGGGCTGTAC CTGCACAGCC TCATCTTCAT GGCCTTCTTC TCAGAGAAGA 1021 AGTACCTGTG GGGCTTCACA GTCTTCGGCT GGGGTGCTGG GACTTGCCCG ACTTACTTGT 1081 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1141 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1201 GAAAGGACGA CTCCAAGACG GCAAAGGCGT AGCAACGCGT TAAGTCgaca atcaacctct 1261 ggattacaaa atttgtgaaa gatt