Construct: ORF TRCN0000474181
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013148.1_s317c1
- Derived from:
- ccsbBroadEn_08782
- DNA Barcode:
- TTTGACCATTAGTGTCGTAATCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNX6 (58533)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474181
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 58533 | SNX6 | sorting nexin 6 | NM_021249.5 | 99.8% | 100% | 309G>A |
| 2 | human | 58533 | SNX6 | sorting nexin 6 | NM_001366519.1 | 78.5% | 78.5% | 1_237del;546G>A |
| 3 | human | 58533 | SNX6 | sorting nexin 6 | NM_152233.4 | 71.3% | 71.4% | 1_348del;657G>A |
| 4 | human | 58533 | SNX6 | sorting nexin 6 | NR_159358.1 | 26.1% | (many diffs) | |
| 5 | human | 58533 | SNX6 | sorting nexin 6 | NR_159357.1 | 25.2% | (many diffs) | |
| 6 | mouse | 72183 | Snx6 | sorting nexin 6 | XM_006516253.3 | 70.9% | 78.3% | (many diffs) |
| 7 | mouse | 72183 | Snx6 | sorting nexin 6 | NM_026998.3 | 64.4% | 71.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 936
- ORF length:
- 870
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac gaaggaagaa ttcacaaaga tgaaacagga actggaagct gaatatttgg 121 caatattcaa gaagacagtt gcgatgcatg aagtgttcct gtgtcgtgtg gcagcacatc 181 ctattttgag aagagattta aatttccatg tcttcttgga atataatcaa gatttgagtg 241 tgcgaggaaa aaataaaaaa gagaaacttg aagacttctt taaaaacatg gttaaatcag 301 cagatggagt aatcgtttca ggagtaaagg atgtagatga tttctttgag cacgaacgaa 361 catttctttt ggaatatcat aaccgagtta aggatgcatc tgctaaatct gatagaatga 421 caagatccca caaaagtgct gcagatgatt acaatagaat tggttcttca ttatatgctt 481 taggaactca ggattctaca gatatatgca agttttttct caaagttTCA GAACTGTTCG 541 ATAAAACAAG AAAAATAGAA GCACGAGTGT CTGCTGATGA AGACCTCAAA CTTTCTGATC 601 TTTTAAAATA TTACTTAAGA GAATCTCAAG CTGCTAAGGA TCTCCTGTAT CGAAGGTCTA 661 GGTCACTAGT GGATTATGAA AATGCTAATA AAGCACTGGA TAAAGCAAGA GCAAAAAATA 721 AAGATGTTCT ACAGGCCGAA ACTTCCCAAC AATTATGTTG TCAGAAATTT GAAAAAATAT 781 CTGAGTCTGC AAAACAAGAA CTTATAGATT TTAAGACAAG AAGAGTTGCT GCATTCAGAA 841 AAAATTTAGT GGAACTGGCA GAGTTAGAAC TGAAGCATGC AAAGGGTAAT CTACAGTTGC 901 TGCAGAACTG CCTGGCAGTG TTAAATGGAG ACACATACCC AACTTTCTTG TACAAAGTGG 961 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1021 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1081 ATTTGACCAT TAGTGTCGTA ATCCCACGCG TTAAGTCgac aatcaacctc tggattacaa 1141 aatttgtgaa agatt