Construct: ORF TRCN0000474182
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009042.1_s317c1
- Derived from:
- ccsbBroadEn_02905
- DNA Barcode:
- ATCCAGACCCGACATCTAAACTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM98 (26022)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474182
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26022 | TMEM98 | transmembrane protein 98 | NM_001033504.1 | 100% | 100% | |
| 2 | human | 26022 | TMEM98 | transmembrane protein 98 | NM_001301746.1 | 100% | 100% | |
| 3 | human | 26022 | TMEM98 | transmembrane protein 98 | NM_015544.3 | 100% | 100% | |
| 4 | mouse | 103743 | Tmem98 | transmembrane protein 98 | NM_029537.1 | 88.4% | 98.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 744
- ORF length:
- 678
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gactgtggtg attgttgcca taggtgtgct ggccaccatc tttctggctt 121 cgtttgcagc cttggtgctg gtttgcaggc agcgctactg ccggccgcga gacctgctgc 181 agcgctatga ttctaagccc attgtggacc tcattggtgc catggagacc cagtctgagc 241 cctctgagtt agaactggac gatgtcgtta tcaccaaccc ccacattgag gccattctgg 301 agaatgaaga ctGGATCGAA GATGCCTCGG GTCTCATGTC CCACTGCATT GCCATCTTGA 361 AGATTTGTCA CACTCTGACA GAGAAGCTTG TTGCCATGAC AATGGGCTCT GGGGCCAAGA 421 TGAAGACTTC AGCCAGTGTC AGCGACATCA TTGTGGTGGC CAAGCGGATC AGCCCCAGGG 481 TGGATGATGT TGTGAAGTCG ATGTACCCTC CGTTGGACCC CAAACTCCTG GACGCACGGA 541 CGACTGCCCT GCTCCTGTCT GTCAGTCACC TGGTGCTGGT GACAAGGAAT GCCTGCCATC 601 TGACGGGAGG CCTGGACTGG ATTGACCAGT CTCTGTCGGC TGCTGAGGAG CATTTGGAAG 661 TCCTTCGAGA AGCAGCCCTA GCTTCTGAGC CAGATAAAGG CCTCCCAGGC CCTGAAGGCT 721 TCCTGCAGGA GCAGTCTGCA ATTTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 781 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 841 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA TCCAGACCCG 901 ACATCTAAAC TCTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 961 att