Construct: ORF TRCN0000474184
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012999.1_s317c1
- Derived from:
- ccsbBroadEn_04590
- DNA Barcode:
- CTGGACCATGTTACGACTAACTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC38A5 (92745)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474184
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92745 | SLC38A5 | solute carrier family 38 me... | NM_033518.4 | 100% | 100% | |
| 2 | human | 92745 | SLC38A5 | solute carrier family 38 me... | XM_017029961.2 | 100% | 100% | |
| 3 | human | 92745 | SLC38A5 | solute carrier family 38 me... | XM_017029960.1 | 98.7% | 98.7% | 1_18del |
| 4 | human | 92745 | SLC38A5 | solute carrier family 38 me... | XM_005272695.4 | 90.9% | 90.9% | 1_141del |
| 5 | human | 92745 | SLC38A5 | solute carrier family 38 me... | XM_005272698.5 | 90% | 88.1% | (many diffs) |
| 6 | human | 92745 | SLC38A5 | solute carrier family 38 me... | XM_006724569.3 | 90% | 88.1% | (many diffs) |
| 7 | human | 92745 | SLC38A5 | solute carrier family 38 me... | XM_005272697.2 | 88.9% | 87.1% | (many diffs) |
| 8 | human | 92745 | SLC38A5 | solute carrier family 38 me... | XM_005272694.3 | 82.3% | 80.5% | (many diffs) |
| 9 | mouse | 209837 | Slc38a5 | solute carrier family 38, m... | NM_172479.3 | 84.6% | 85.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1482
- ORF length:
- 1416
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga actgcaggat ccaaagatga atggagccct cccttcggat gctgtgggct 121 acaggcaaga acgtgagggc ttcctgccca gtcgtggtcc tgctcctggg agcaagccgg 181 tccagttcat ggatttcgag gggaagacat cgtttggaat gtcagtgttc aacctcagca 241 acgccatcat gggcagcggc atcctggggc tggcctatgc catggcccac acgggggtca 301 tcttcttcct ggccctgctg ctgtgcattg cgcttctgtc gtcctactcc atccacctcc 361 tgctgacctg tgctggtatt gcaggcatcc gagcctatga gcagctggga cagagggcat 421 tcgggcctgc ggggaaggta gtggtggcca cagtcatctg tctgcacaat gttggggcca 481 tgtccagtta cctgttcatc atcaaatctg agctccccct ggttatcggc accttcctgt 541 acatggaccc cgagggggac tggttcttga agggaaacct cctcatcatc atcgtcagtg 601 tgttaatcat cctgcccctc gccctcatga aacacttggg ctacctgggg tacaccagtg 661 gtctctctct gacctgcatg ctgtttttcc ttgtttcggt catctacaag aagttccaac 721 ttggctgtgc tataggccac aatgaaacag caatggagag tgaagctctc gtgggactcc 781 ccagccaagg actcaacagc agctgtgagg cccagatgtt cacagttgac tcacagatgt 841 cctacacagt gcccattatg gcttttgctt ttgtctgcca ccctgaggtg ctgcccatct 901 atacggagct ctgccGGCCC TCCAAGCGCA GGATGCAGGC CGTGGCCAAC GTGTCCATTG 961 GGGCCATGTT CTGCATGTAT GGGCTCACAG CAACCTTTGG ATACCTCACC TTCTACAGCA 1021 GTGTGAAGGC GGAGATGCTG CACATGTACA GCCAGAAGGA CCCGCTCATC CTCTGTGTGC 1081 GCCTGGCCGT GCTGCTCGCG GTGACCCTCA CTGTGCCAGT CGTGCTGTTC CCTATCCGCC 1141 GGGCCCTGCA GCAGCTGCTT TTCCCAGGCA AGGCCTTCAG CTGGCCACGA CATGTGGCCA 1201 TAGCTCTGAT CCTGCTTGTT TTGGTCAATG TCCTTGTCAT CTGTGTGCCA ACCATCCGGG 1261 ATATCTTTGG AGTTATCGGG TCCACCTCAG CCCCCAGCCT CATCTTCATC CTCCCCAGCA 1321 TCTTCTACCT CCGCATTGTA CCCTCTGAGG TGGAGCCTTT CTTATCCTGG CCCAAGATCC 1381 AGGCCCTGTG CTTTGGAGTC CTGGGAGTCC TCTTCATGGC CGTCAGTCTA GGCTTTATGT 1441 TTGCCAACTG GGCCACAGGC CAGAGCCGCA TGTCTGGACA CTGCCCAACT TTCTTGTACA 1501 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1561 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1621 AGGACGACTG GACCATGTTA CGACTAACTT TACGCGTTAA GTCgacaatc aacctctgga 1681 ttacaaaatt tgtgaaagat t