Construct: ORF TRCN0000474204
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001039.1_s317c1
- Derived from:
- ccsbBroadEn_08898
- DNA Barcode:
- ACGGCAACGGTGATGGCTCCCGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRAK2 (66008)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474204
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 66008 | TRAK2 | trafficking kinesin protein 2 | NM_015049.3 | 99.7% | 99.4% | (many diffs) |
| 2 | human | 66008 | TRAK2 | trafficking kinesin protein 2 | XM_011511690.1 | 99.7% | 99.4% | (many diffs) |
| 3 | human | 66008 | TRAK2 | trafficking kinesin protein 2 | XM_017004772.1 | 76.7% | 76.4% | (many diffs) |
| 4 | mouse | 70827 | Trak2 | trafficking protein, kinesi... | NM_172406.3 | 84.9% | 86.9% | (many diffs) |
| 5 | mouse | 70827 | Trak2 | trafficking protein, kinesi... | XM_011238600.2 | 84.9% | 86.9% | (many diffs) |
| 6 | mouse | 70827 | Trak2 | trafficking protein, kinesi... | XM_006496280.2 | 84.7% | 86.8% | (many diffs) |
| 7 | mouse | 70827 | Trak2 | trafficking protein, kinesi... | XM_006496281.3 | 78.8% | 80.9% | (many diffs) |
| 8 | mouse | 70827 | Trak2 | trafficking protein, kinesi... | XM_006496282.2 | 62.8% | 62.5% | (many diffs) |
| 9 | mouse | 70827 | Trak2 | trafficking protein, kinesi... | XM_017312290.1 | 59.7% | 60.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2808
- ORF length:
- 2742
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tcaatcccag aatgcaattt ttacatcacc aacaggtgaa gaaaacctca 121 tgaatagcaa tcacagagac tcggagagca tcactgatgt ctgctccaat gaggatctcc 181 ctgaagttga gctggtgagt ctgctagaag aacaactacc acagtatagg ctaaaagtag 241 acactctctt tctatatgaa aatcaagact ggactcagtc tccacaccag cggcagcatg 301 catctgatgc tctctctcca gtccttgctg aagagacttt ccgttacatg attctaggca 361 cagacagggt ggagcagatg accaaaactt acaatgacat cgacatggtt acacatctcc 421 tggcagagag ggatcgtgat ctggaactcg ctgctcgaat tggacaagct ctcttaaagc 481 ggaaccatat cttatctgag cagaacgaat ccctggagga gcaattggga caagcctttg 541 atcaagttaa tcagctgcag catgagctat gcaagaaaga tgagttactt cgaatcgtct 601 ccattgcttc tgaagaaagt gaaactgatt ccagctgttc tacacctctt cggttcaatg 661 agtcctttag cttatctcaa gggttgctgc agttggaaat gctgcaagaa aagctcaagg 721 aactggaaga agagaatatg gctcttcgat ccaaggcttg tcacataaag acagaaactg 781 ttacctatga agaaaaggaa caacagcttg tcagcgactg tgttaaagaa cttcgtgaaa 841 caaatgctca gatgtccaga atgactgaag aattgtcagg gaagagtgat gagctggttc 901 gataccaaga agagctttcc tctcttttgt cacagattgt agaccttcag cataaactta 961 aagaacatgt gattgagaag gaagaactaa aacttcacct gcaagcttcc aaagatgccc 1021 aacggcaact gacaatggag ctgcacgagt tacaagacag gaatatggag tgtctaggaa 1081 tgttacatga atcccaagaa gaaataaagg aacttcgtag tagatctggc cctactgctc 1141 atctctactt ctcccaatca tatggagctt ttactgggga atctttggca gctgagattg 1201 aggggactat gcgtaaaaag ctgagtttgg atgaggaatc ttctctcttt aaacaaaaag 1261 cccaacagaa gcgggtattt gataccgtca ggattgccaa tgacacacgg ggccgctcta 1321 tctcattccc agctctgtta cccattccag gctccaaccg ttcaagtgtc atcatgacag 1381 caaaaccttt tgagtctggt cttcagcaaa cagaggacaa atcactcctg aaccagggga 1441 gcagctcaga ggaggttgca gggagctccc agaagatggg ccaaccagga ccctcaggag 1501 atagtgattt ggctacagca ctgcatcgcc ttagcttgcg tcgacaaaac tatttaagtg 1561 agaagcagtt ctttgctgaa gaatggcagc ggaagatcca ggttctggca gaccagaagg 1621 aaggagttag tggctgtgtc accccgacag agagccttgc ctctctctgc accacccagt 1681 cagagatcac agacctcagc agtgccagtt gccttcgagg ttttatgcca gaaaaattac 1741 aaattgtcaa gccccttgaa ggatcacaaa ctctgtatca ctggcagcag cttgctcaac 1801 caaacttggg aaccatcctt gatcaacgac caggtgtcat tactaaaggc tttacccagt 1861 tgcccgggga tgctatttat cacatctcag atttagaaga ggatgaagag gagggtatta 1921 cttttcaggt tcagcaacct cttgaagtgg aagagaaact ttcaacatcc aagccagtaa 1981 cagggatctt cctgccaccc attacttcag caggtggacc agttacagtt gcaaccgcca 2041 gcccaggaaa gtgcctgtcg tgcacaaact caacattcac tttcaccacc tgtagaatat 2101 tacatccctc tgacatcact caggttaccc ccagctctgg gttcccttca ttatcctgtg 2161 gaagtagcgg tagcagttca tccaacacgg ctgtgaattc tcctgccttg tcctatagac 2221 tcagcattgg tgagtccatc accaaccgac gagattccac tacaaccttc agtagcacca 2281 tgagcttggc caaacttcta caagagcgag gcatctctgc caaagtgtac cacagcccaa 2341 tttcagagaa ccccctccag cctctcccta aatccctggc tatcccttcc acaccaccaa 2401 attcaccatc tcactcacct tgcccttctc ctttaccctt tgagcctcga gtgcatctct 2461 ctgaaaattt tttggcctct cgaccagctg agacattcct ccaggagatg tatggcttga 2521 gacactcccg gaaccctccT GATGTTGGCC AGTTGAAGAT GAACTTAGTG GACAGGCTGA 2581 AGAGACTGGG GATAGCCAGA GTGGTCAAGA ACCCTGGTGC CCAAGAGAAT GGAAGATGCC 2641 AGGAGGCAGA AATTGGTCCT CAAAAACCAG ATTCTGCTGT TTATTTAAAT TCAGGTAGCA 2701 GTTTATTAGG TGGACTAAGG AGGAATCAGA GTCTTCCAGT CATAATGGGT AGCTTTGCTG 2761 CCCCAGTTTG CACATCCTCA CCCAAAATGG GTGTCCTGAA GGAGGACTGC CCAACTTTCT 2821 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2881 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2941 GTGGAAAGGA CGAACGGCAA CGGTGATGGC TCCCGGCACG CGTTAAGTCg acaatcaacc 3001 tctggattac aaaatttgtg aaagatt