Construct: ORF TRCN0000474216
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016832.1_s317c1
- Derived from:
- ccsbBroadEn_02424
- DNA Barcode:
- TTACAGCTCTGTTCCGTCCGGCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBAC1 (10422)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474216
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10422 | UBAC1 | UBA domain containing 1 | NM_016172.3 | 99.9% | 100% | 519A>G |
| 2 | mouse | 98766 | Ubac1 | ubiquitin associated domain... | NM_133835.2 | 84.4% | 89.5% | (many diffs) |
| 3 | mouse | 98766 | Ubac1 | ubiquitin associated domain... | XM_006498464.1 | 70% | 69.8% | (many diffs) |
| 4 | mouse | 98766 | Ubac1 | ubiquitin associated domain... | XM_006498465.1 | 63.6% | 61.8% | (many diffs) |
| 5 | mouse | 98766 | Ubac1 | ubiquitin associated domain... | XM_011239219.2 | 62.8% | 66.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1281
- ORF length:
- 1215
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt cgtgcaggag gagaagatct tcgcgggcaa ggtgctgcgg ctgcacatct 121 gcgcgtccga cggcgccgag tggctggagg aggccaccga ggacacctcg gtggagaagc 181 tcaaggagcg ctgcctcaag cactgtgctc atgggagctt agaagatccc aaaagtataa 241 cccatcataa attaatccac gctgcctcag agagggtgct gagtgatgcc aggaccatcc 301 tggaagagaa catccaggac caagatgtcc tattattgat aaaaaagcgt gctccatcac 361 cacttcccaa gatggctgat gtctcagcag aagaaaagaa aaaacaagac cagaaagctc 421 cagataaaga ggccatactg cgggccaccg ccaacctgcc ctcctacaac atggaccggg 481 ccgcggtcca gaccaacatg agagacttcc agacagaact ccggaagata ctggtgtctc 541 tcatcgaggt ggcgcagaag ctgttagcgc tgaacccaga tgcggtggaa ttgtttaaga 601 aggcgaatgc aatgctggac gaggacgagg atgagcgtgt ggacgaggct gccctgcggc 661 agctcacgga gatgggcttt ccggagaaca gagccaccaa ggcccttcag ctgaaccaca 721 tgtcggtgcc tcaggccatg gagtggctaa ttgaacacgc agaagacccg accatagaca 781 cgcctcttcc tggccaagct cccccagagg ccgagggggc cacagcagct gccTCCGAGG 841 CTGCCGCGGG AGCCAGCGCC ACCGATGAGG AGGCCAGAGA TGAGCTGACG GAAATCTTCA 901 AGAAGATCCG GAGGAAAAGG GAGTTTCGGG CTGATGCTCG GGCCGTCATT TCCCTGATGG 961 AGATGGGGTT CGACGAGAAA GAGGTGATAG ATGCCCTCAG AGTGAACAAC AACCAGCAGA 1021 ATGCCGCGTG CGAGTGGCTG CTGGGGGACC GGAAGCCCTC TCCGGAGGAG CTGGACAAGG 1081 GCATCGACCC CGACAGTCCT CTCTTTCAGG CCATCCTGGA TAACCCGGTG GTGCAGCTGG 1141 GCCTGACCAA CCCGAAAACA TTGCTAGCAT TTGAAGACAT GCTGGAGAAC CCACTGAACA 1201 GCACCCAGTG GATGAATGAT CCAGAAACGG GGCCTGTCAT GCTGCAGATC TCTAGAATCT 1261 TCCAGACACT AAATCGCACG TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1321 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1381 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATTAC AGCTCTGTTC 1441 CGTCCGGCTA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt