Construct: ORF TRCN0000474218
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017189.1_s317c1
- Derived from:
- ccsbBroadEn_04067
- DNA Barcode:
- CCTGTACGTTCTTAGATCACAACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- THTPA (79178)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474218
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79178 | THTPA | thiamine triphosphatase | NM_001126339.3 | 100% | 100% | |
| 2 | human | 79178 | THTPA | thiamine triphosphatase | NM_024328.6 | 100% | 100% | |
| 3 | human | 79178 | THTPA | thiamine triphosphatase | NM_001256322.2 | 60.8% | 60.8% | 277_278ins270 |
| 4 | human | 79178 | THTPA | thiamine triphosphatase | NM_001256323.2 | 60.8% | 60.8% | 277_278ins270 |
| 5 | human | 79178 | THTPA | thiamine triphosphatase | NM_001256062.2 | 44.5% | 41.3% | (many diffs) |
| 6 | human | 79178 | THTPA | thiamine triphosphatase | NM_001256321.2 | 44.5% | 41.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 756
- ORF length:
- 690
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 tcggcatggc ccagggcttg attgaggtgg agcgaaagtt ccttccaggg cctggcacag 121 aggagcggct gcaggagttg gggggcaccc tggagtaccg ggtcaccttc cgagacacct 181 actatgacac ccctgagctg agcctcatgc aggctgacca ctggctgcga cgacgagagg 241 atagtggatg ggagctcaaa tgtcctGGAG CAGCAGGTGT CTTAGGACCC CACACGGAGT 301 ATAAGGAACT CACAGCGGAA CCTACAATTG TGGCCCAACT CTGTAAGGTG CTGCGGGCTG 361 ACGGCCTGGG GGCTGGAGAT GTGGCTGCTG TGCTGGGCCC ACTGGGGCTG CAGGAAGTAG 421 CTAGTTTTGT GACTAAGCGG AGTGCCTGGA AGCTGGTGCT CTTGGGAGCT GATGAAGAGG 481 AGCCACAGCT CAGGGTGGAC TTGGATACAG CCGACTTTGG CTACGCTGTG GGTGAGGTAG 541 AGGCCCTGGT GCATGAGGAG GCTGAAGTAC CAACTGCCCT AGAGAAGATC CACAGGCTCA 601 GCAGCATGCT TGGTGTGCCT GCACAGGAGA CAGCACCAGC CAAGCTGATT GTGTATCTAC 661 AGCGTTTCCG GCCTCAAGAC TATCAGCGCC TGCTAGAAGT GAACAGCTCC AGAGAGAGGC 721 CACAGGAGAC TGAAGATCCT GACCACTGCC TGGGCTACCC AACTTTCTTG TACAAAGTGG 781 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 841 CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CCTGTACGTT 901 CTTAGATCAC AACTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa 961 gatt