Transcript: Human NM_001256322.2

Homo sapiens thiamine triphosphatase (THTPA), transcript variant 6, mRNA.

Source:
NCBI, updated 2019-02-24
Taxon:
Homo sapiens (human)
Gene:
THTPA (79178)
Length:
1958
CDS:
777..1199

Additional Resources:

NCBI RefSeq record:
NM_001256322.2
NBCI Gene record:
THTPA (79178)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001256322.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000271651 TTAGCCGTGATCAGTAGTTAA pLKO_005 1472 3UTR 100% 13.200 18.480 N THTPA n/a
2 TRCN0000201985 CCATTGATTTCCGCTCGTGTT pLKO.1 1437 3UTR 100% 4.050 5.670 N Thtpa n/a
3 TRCN0000271602 GTTTCCGGCCTCAAGACTATC pLKO_005 1105 CDS 100% 10.800 8.640 N THTPA n/a
4 TRCN0000281814 ATAGTGGATGGGAGCTCAAAT pLKO_005 952 CDS 100% 13.200 9.240 N THTPA n/a
5 TRCN0000051078 GCCAAGCTGATTGTGTATCTA pLKO.1 1080 CDS 100% 5.625 3.938 N THTPA n/a
6 TRCN0000051082 GAACCTACAATTGTGGCCCAA pLKO.1 1029 CDS 100% 2.160 1.512 N THTPA n/a
7 TRCN0000051081 ACCTTCCGAGACACCTACTAT pLKO.1 876 CDS 100% 5.625 3.375 N THTPA n/a
8 TRCN0000190064 GAGCTCAAATGTCCTGGAGTA pLKO.1 963 CDS 100% 4.050 2.835 N Thtpa n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001256322.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04067 pDONR223 100% 60.8% 60.8% None 277_278ins270 n/a
2 ccsbBroad304_04067 pLX_304 0% 60.8% 60.8% V5 277_278ins270 n/a
3 TRCN0000474218 CCTGTACGTTCTTAGATCACAACT pLX_317 70.4% 60.8% 60.8% V5 277_278ins270 n/a
Download CSV