Construct: ORF TRCN0000474227
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007544.1_s317c1
- Derived from:
- ccsbBroadEn_06492
- DNA Barcode:
- TACTGTATTCCGACACTGACTGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLKB1 (3818)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474227
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3818 | KLKB1 | kallikrein B1 | NM_000892.5 | 99.8% | 99.6% | 428G>A;1679G>A;1761T>C |
| 2 | human | 3818 | KLKB1 | kallikrein B1 | XM_011531930.2 | 98.1% | 97.9% | (many diffs) |
| 3 | human | 3818 | KLKB1 | kallikrein B1 | XM_017008181.1 | 98.1% | 97.9% | (many diffs) |
| 4 | human | 3818 | KLKB1 | kallikrein B1 | XM_017008183.1 | 86.4% | 82.5% | (many diffs) |
| 5 | human | 3818 | KLKB1 | kallikrein B1 | XM_017008182.1 | 84.9% | 81.1% | (many diffs) |
| 6 | human | 3818 | KLKB1 | kallikrein B1 | NM_001318394.2 | 80.4% | 76.5% | (many diffs) |
| 7 | human | 3818 | KLKB1 | kallikrein B1 | NM_001318396.2 | 65.5% | 61.6% | (many diffs) |
| 8 | human | 3818 | KLKB1 | kallikrein B1 | XM_017008184.1 | 65.5% | 61.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1983
- ORF length:
- 1914
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gattttattc aagcaagcaa cttatttcat ttccttgttt gctacagttt 121 cctgtggatg tctgactcaa ctctatgaaa acgccttctt cagaggtggg gatgtagctt 181 ccatgtacac cccaaatgcc caatactgcc agatgaggtg cacattccac ccaaggtgtt 241 tgctattcag ttttcttcca gcaagttcaa tcaatgacat ggagaaaagg tttggttgct 301 tcttgaaaga tagtgttaca ggaaccctgc caaaagtaca tcgaacaggt gcagtttctg 361 gacattcctt gaagcaatgt ggtcatcaaa taagtgcttg ccatcgagac atttataaag 421 gagttgatat gagaggagtc aattttaatg tgtctaaggt tagcagtgtt gaagaatgcc 481 aaaaaaggtg caccaataac attcgctgcc agtttttttc atatgccacg caaacatttc 541 acaaggcaga gtaccggaac aattgcctat taaagtacag tcccggagga acacctaccg 601 ctataaaggt gctgagtaac gtggaatctg gattctcact gaagccctgt gccctttcag 661 aaattggttg ccacatgaac atcttccagc atcttgcgtt ctcagatgtg gatgttgcca 721 gggttctcac tccagatgct tttgtgtgtc ggaccatctg cacctatcac cccaactgcc 781 tcttctttac attctataca aatgtatgga aaatcgagtc acaaagaaat gtttgtcttc 841 ttaaaacatc tgaaagtggc acaccaagtt cctctactcc tcaagaaaac accatatctg 901 gatatagcct tttaacctgc aaaagaactt tacctgaacc ctgccattct aaaatttacc 961 cgggagttga ctttggagga gaagaattga atgtgacttt tgttaaagga gtgaatgttt 1021 gccaagagac ttgcacaaag atgattcgct gtcagttttt cacttattct ttactcccag 1081 aagactgtaa ggaagagaag tgtaagtgtt tcttaagatt atctatggat ggttctccaa 1141 ctaggattgc gtatgggaca caagggagct ctggttactc tttgagattg tgtaacactg 1201 gggacaactc tgtctgcaca acaaaaacaa gcacacgcat tgttggagga acaaactctt 1261 cttggggaga gtggccctgg caggtgagcc tgcaggtgaa gctgacagct cagaggcacc 1321 tgtgtggagg gtcactcata ggacaccagt gggtcctcac tgctgcccac tgctttgatg 1381 ggcttcccct gcaggatgtt tggcgcatct atagtggcat tttaaatctg tcagacatta 1441 caaaagatac acctttctca caaataaaag agattattat tcaccaaaac tataaagtct 1501 cagaagggaa tcatgatatc gccttgataa aactccaggc tcctttgaat tacactgaat 1561 tccaaaaacc aatatgccta ccttccaaag gtgacacaag cacaatttat accaactgtt 1621 gggTAACCGG ATGGGGCTTC TCGAAGGAGA AAGGTGAAAT CCAAAATATT CTACAAAAGG 1681 TAAATATTCC TTTGGTAACA AATGAAGAAT GCCAGAAAAG ATATCAAGAT TATAAAATAA 1741 CCCAACAGAT GGTCTGTGCT GGCTATAAAG AAGGGGGAAA AGATGCTTGT AAGGGAGATT 1801 CAGGTGGTCC CTTAGTTTGC AAACACAACG GAATGTGGCG TTTGGTGGGC ATCACCAGCT 1861 GGGGTGAAGG CTGTGCCCGC AGGGAGCAAC CTGGTGTCTA CACCAAAGTC GCTGAGTACA 1921 TGGACTGGAT TTTAGAGAAA ACACAGAGCA GTGATGGAAA AGCTCAGATG CAGTCACCAG 1981 CATTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2041 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2101 GGCTTTATAT ATCTTGTGGA AAGGACGATA CTGTATTCCG ACACTGACTG TCACGCGTTA 2161 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt