Construct: ORF TRCN0000474237
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006617.1_s317c1
- Derived from:
- ccsbBroadEn_10788
- DNA Barcode:
- AATGCATTGCCCTCAATCATTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RCAN1 (1827)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474237
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1827 | RCAN1 | regulator of calcineurin 1 | NM_203418.3 | 90.1% | 88.8% | (many diffs) |
| 2 | human | 1827 | RCAN1 | regulator of calcineurin 1 | NM_001285389.2 | 86.1% | 85.2% | (many diffs) |
| 3 | human | 1827 | RCAN1 | regulator of calcineurin 1 | NM_004414.7 | 78.1% | 78.1% | 1_165del |
| 4 | human | 1827 | RCAN1 | regulator of calcineurin 1 | NM_001285391.2 | 68.6% | 61.6% | (many diffs) |
| 5 | human | 1827 | RCAN1 | regulator of calcineurin 1 | XM_017028283.1 | 68.6% | 61.6% | (many diffs) |
| 6 | human | 1827 | RCAN1 | regulator of calcineurin 1 | NM_001331016.1 | 62.1% | 54.3% | (many diffs) |
| 7 | human | 1827 | RCAN1 | regulator of calcineurin 1 | NM_001285392.2 | 59.3% | 59.3% | 0_1ins240 |
| 8 | human | 1827 | RCAN1 | regulator of calcineurin 1 | NM_001285393.2 | 59.3% | 59.3% | 0_1ins240 |
| 9 | human | 1827 | RCAN1 | regulator of calcineurin 1 | NM_203417.2 | 59.3% | 59.3% | 0_1ins240 |
| 10 | human | 1827 | RCAN1 | regulator of calcineurin 1 | XM_005260929.2 | 56.2% | 51.5% | (many diffs) |
| 11 | human | 1827 | RCAN1 | regulator of calcineurin 1 | XM_017028282.2 | 49.6% | 39.1% | (many diffs) |
| 12 | mouse | 54720 | Rcan1 | regulator of calcineurin 1 | NM_019466.4 | 76.8% | 86.8% | (many diffs) |
| 13 | mouse | 54720 | Rcan1 | regulator of calcineurin 1 | NM_001081549.2 | 70.3% | 76.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 657
- ORF length:
- 591
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggaggtggac ctgcaggacc tgcccagcgc caccatcgcc tgtcacctGG 121 ACCCGCGCGT GTTCGTGGAC GGCCTGTGCC GGGCCAAATT TGAGTCCCTC TTTAGGACGT 181 ATGACAAGGA CATCACCTTT CAGTATTTTA AGAGCTTCAA ACGAGTCAGA ATAAACTTCA 241 GCAACCCCTT CTCCGCAGCA GATGCCAGGC TCCAGCTGCA TAAGACTGAG TTTCTGGGAA 301 AGGAAATGAA GTTATATTTT GCTCAGACCT TACACATAGG AAGCTCACAC CTGGCTCCGC 361 CAAATCCAGA CAAGCAGTTT CTGATCTCCC CTCCCGCCTC TCCGCCAGTG GGATGGAAAC 421 AAGTGGAAGA TGCGACCCCA GTCATAAACT ATGATCTCTT ATATGCCATC TCCAAGCTGG 481 GGCCAGGGGA AAAGTATGAA TTGCACGCAG CGACTGACAC CACTCCCAGC GTGGTGGTCC 541 ATGTATGTGA GAGTGATCAA GAGAAGGAGG AAGAAGAGGA AATGGAAAGA ATGAGGAGAC 601 CTAAGCCAAA AATTATCCAG ACCAGGAGGC CGGAGTACAC GCCGATCCAC CTCAGCTGCC 661 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 721 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 781 ATATATCTTG TGGAAAGGAC GAAATGCATT GCCCTCAATC ATTTTCACGC GTTAAGTCga 841 caatcaacct ctggattaca aaatttgtga aagatt