Construct: ORF TRCN0000474238
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015437.1_s317c1
- Derived from:
- ccsbBroadEn_08493
- DNA Barcode:
- TCCAGCTGTTCCCGATGATCGTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LRRC20 (55222)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474238
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55222 | LRRC20 | leucine rich repeat contain... | NM_001278211.2 | 99.8% | 100% | 180C>T |
| 2 | human | 55222 | LRRC20 | leucine rich repeat contain... | NM_001278212.2 | 99.8% | 100% | 180C>T |
| 3 | human | 55222 | LRRC20 | leucine rich repeat contain... | NM_207119.3 | 99.8% | 100% | 180C>T |
| 4 | human | 55222 | LRRC20 | leucine rich repeat contain... | XM_017016374.1 | 99.8% | 100% | 180C>T |
| 5 | human | 55222 | LRRC20 | leucine rich repeat contain... | XM_017016375.1 | 99.8% | 100% | 180C>T |
| 6 | human | 55222 | LRRC20 | leucine rich repeat contain... | NM_001278213.1 | 72.8% | 72.8% | 81_82ins150 |
| 7 | human | 55222 | LRRC20 | leucine rich repeat contain... | NM_018239.4 | 72.8% | 72.8% | 81_82ins150 |
| 8 | human | 55222 | LRRC20 | leucine rich repeat contain... | NM_018205.4 | 69.3% | 69.5% | 180C>T;230_231ins168 |
| 9 | human | 55222 | LRRC20 | leucine rich repeat contain... | NM_001278214.1 | 62.5% | 62.5% | 0_1ins207 |
| 10 | human | 55222 | LRRC20 | leucine rich repeat contain... | NR_103467.2 | 12.6% | (many diffs) | |
| 11 | mouse | 216011 | Lrrc20 | leucine rich repeat contain... | NM_153542.1 | 85% | 89.6% | (many diffs) |
| 12 | mouse | 216011 | Lrrc20 | leucine rich repeat contain... | XM_017313884.1 | 61.8% | 64.6% | (many diffs) |
| 13 | mouse | 216011 | Lrrc20 | leucine rich repeat contain... | XM_017313885.1 | 59.7% | 63% | (many diffs) |
| 14 | mouse | 216011 | Lrrc20 | leucine rich repeat contain... | XM_006513472.3 | 52.3% | 54.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 618
- ORF length:
- 552
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gaagaagatg ggtgaggccg tggccagagt agcaaggaag gtcaacgaga 121 cggtggagag cggcTCTGAC ACTCTGGACC TGGCCGAGTG CAAGCTGGTC TCCTTTCCCA 181 TTGGCATCTA CAAGGTCCTG CGGAATGTCT CTGGCCAGAT CCACCTCATC ACCCTGGCTA 241 ACAATGAGCT TAAGTCCCTC ACCAGCAAGT TCATGACCAC ATTCAGTCAG CTCCGAGAGC 301 TCCACCTGGA GGGGAACTTC CTACACCGCC TCCCCAGCGA GGTCAGTGCC CTGCAGCACC 361 TCAAGGCCAT TGACCTGTCC CGGAACCAGT TCCAGGACTT CCCTGAGCAG CTTACCGCCC 421 TGCCGGCGCT GGAGACCATC AACCTGGAGG AGAACGAGAT CGTAGATGTG CCCGTGGAGA 481 AGCTGGCCGC CATGCCAGCC TTGCGCAGCA TCAACCTCCG CTTCAACCCA CTCAACGCCG 541 AGGTGCGCGT GATCGCCCCG CCGCTCATCA AGTTTGACAT GCTCATGTCT CCGGAAGGCG 601 CAAGAGCCCC CCTACCTTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 661 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 721 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATCCAGCT GTTCCCGATG 781 ATCGTCTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt