Construct: ORF TRCN0000474248
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003091.1_s317c1
- Derived from:
- ccsbBroadEn_11704
- DNA Barcode:
- CCCCCCGAGCAAGAAGATTAATGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANKRD12 (23253)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474248
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23253 | ANKRD12 | ankyrin repeat domain 12 | XM_011525638.3 | 16.9% | 16.4% | (many diffs) |
| 2 | human | 23253 | ANKRD12 | ankyrin repeat domain 12 | NM_015208.5 | 15.8% | 15.3% | (many diffs) |
| 3 | human | 23253 | ANKRD12 | ankyrin repeat domain 12 | XM_005258092.4 | 15.8% | 15.3% | (many diffs) |
| 4 | human | 23253 | ANKRD12 | ankyrin repeat domain 12 | XM_005258093.4 | 15.8% | 15.3% | (many diffs) |
| 5 | human | 23253 | ANKRD12 | ankyrin repeat domain 12 | XM_024451107.1 | 15.8% | 15.3% | (many diffs) |
| 6 | human | 23253 | ANKRD12 | ankyrin repeat domain 12 | XM_005258095.3 | 15.7% | 15.2% | (many diffs) |
| 7 | human | 23253 | ANKRD12 | ankyrin repeat domain 12 | XM_017025661.2 | 15.7% | 15.2% | (many diffs) |
| 8 | human | 23253 | ANKRD12 | ankyrin repeat domain 12 | XM_017025662.2 | 15.7% | 15.2% | (many diffs) |
| 9 | human | 23253 | ANKRD12 | ankyrin repeat domain 12 | NM_001083625.3 | 14.6% | 14.2% | (many diffs) |
| 10 | human | 23253 | ANKRD12 | ankyrin repeat domain 12 | NM_001204056.1 | 14.6% | 14.2% | (many diffs) |
| 11 | human | 23253 | ANKRD12 | ankyrin repeat domain 12 | XM_017025663.2 | 14.6% | 14.2% | (many diffs) |
| 12 | human | 23253 | ANKRD12 | ankyrin repeat domain 12 | XM_005258096.3 | 14.5% | 14% | (many diffs) |
| 13 | human | 23253 | ANKRD12 | ankyrin repeat domain 12 | XM_017025664.2 | 14.5% | 14% | (many diffs) |
| 14 | human | 23253 | ANKRD12 | ankyrin repeat domain 12 | XM_017025665.2 | 14.5% | 14% | (many diffs) |
| 15 | human | 23253 | ANKRD12 | ankyrin repeat domain 12 | XM_017025666.2 | 14.5% | 14% | (many diffs) |
| 16 | mouse | 106585 | Ankrd12 | ankyrin repeat domain 12 | NM_001025572.1 | 14.3% | 14.7% | (many diffs) |
| 17 | mouse | 106585 | Ankrd12 | ankyrin repeat domain 12 | XM_006523396.3 | 14.2% | 14.6% | (many diffs) |
| 18 | mouse | 106585 | Ankrd12 | ankyrin repeat domain 12 | XM_006523393.3 | 14.1% | 14.5% | (many diffs) |
| 19 | mouse | 106585 | Ankrd12 | ankyrin repeat domain 12 | XM_006523394.3 | 14.1% | 14.5% | (many diffs) |
| 20 | mouse | 106585 | Ankrd12 | ankyrin repeat domain 12 | XM_006523395.3 | 14.1% | 14.5% | (many diffs) |
| 21 | mouse | 106585 | Ankrd12 | ankyrin repeat domain 12 | XM_006523399.3 | 13.2% | 13.6% | (many diffs) |
| 22 | mouse | 106585 | Ankrd12 | ankyrin repeat domain 12 | XM_006523397.3 | 13% | 13.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1056
- ORF length:
- 990
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc caaatctggg ttcacaaaac caattcagag tgaaaattct gacagtgaca 121 gcaatatggt agagaaacca tatggaagaa agagtaaaga caagattgca tcctacagca 181 aaactccaaa aattgaacga agtgatgtga gcaaggagat gaaagagaaa tcatccatga 241 aacgtaaact tccttttact attagcccat caagaaatga agaacgagat tcagacacag 301 attcagatcc aggacataca agtgaaaatt ggggggagag acttatatct tcttacagga 361 catactcaga gaaagaaggt ccagaaaaga agaagacaaa aaaggaagct ggaaataaga 421 aatccacacc agttagcatt ctttttggtt atccactctc tgagcgaaaa cagatggcac 481 ttcttatgca gatgacagca agagacaaca gtccagattc cacaccaaat catccatcac 541 aaacaacgcc tgcccaaaag aaaactccca gttcTTCATC TCGACAGAAA GATAAAGTTA 601 ATAAAAGAAA TGAACGTGGT GAAACTCCTT TACACATGGC TGCTATTCGA GGAGATGTGA 661 AACAAGTTAA AGAATTAATA AGTTTAGGGG CAAATGTGAA TGTGAAAGAT TTTGCAGGTT 721 GGACACCACT GCATGAAGCT TGCAATGTTG GATATTACGA TGTTGCTAAG ATACTTATAG 781 CAGCTGGAGC AGATGTTAAC ACACAAGGAT TAGATGATGA CACTCCACTC CATGATTCTG 841 CTAGTAGTGG GCACAGAGAT ATAGTAAAGC TGTTACTTCG TCACGGTGGA AATCCATTTC 901 AAGCTAATAA ACATGGGGAG CGTCCAGTGG ATGTAGCAGA AACAGAGGAG TTGGAGTTGC 961 TACTAAAAAG AGAGGTGCCT TTATCTGATG ATGATGAAAG TTACACAGGA TCCAGCTACC 1021 AATTTGCAGG AAATGCCAAG CTCAGCGGAG CAAGTTGCCC AACTTTCTTG TACAAAGTGG 1081 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1141 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1201 ACCCCCCGAG CAAGAAGATT AATGCACGCG TTAAGTCgac aatcaacctc tggattacaa 1261 aatttgtgaa agatt