Transcript: Human XM_005258095.3

PREDICTED: Homo sapiens ankyrin repeat domain 12 (ANKRD12), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ANKRD12 (23253)
Length:
8440
CDS:
241..6423

Additional Resources:

NCBI RefSeq record:
XM_005258095.3
NBCI Gene record:
ANKRD12 (23253)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005258095.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000425266 AGCAATCAAGGTAGCTTATTA pLKO_005 4222 CDS 100% 15.000 21.000 N ANKRD12 n/a
2 TRCN0000417992 GACTTGCCGGAGCGGATTAAA pLKO_005 4036 CDS 100% 15.000 21.000 N ANKRD12 n/a
3 TRCN0000127737 GCTAATCAAGAGCCAGGTATT pLKO.1 4777 CDS 100% 10.800 15.120 N ANKRD12 n/a
4 TRCN0000127761 GCACTGGTTCTGTTGAAGTTA pLKO.1 8284 3UTR 100% 5.625 7.875 N ANKRD12 n/a
5 TRCN0000129083 GCAAGAACATTGGCAAATCAA pLKO.1 6052 CDS 100% 5.625 4.500 N ANKRD12 n/a
6 TRCN0000130913 GCTGCTGAACTTGTTAGACAT pLKO.1 7837 3UTR 100% 4.950 3.960 N ANKRD12 n/a
7 TRCN0000421513 AGTGCCATTGAGGAATCTATA pLKO_005 2605 CDS 100% 13.200 9.240 N ANKRD12 n/a
8 TRCN0000419004 GAAGATCATAGTCCAACATTT pLKO_005 2113 CDS 100% 13.200 9.240 N ANKRD12 n/a
9 TRCN0000128899 GCAAGTATGTTTCAGCTGATA pLKO.1 4370 CDS 100% 4.950 3.465 N ANKRD12 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005258095.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11704 pDONR223 100% 15.7% 15.2% None (many diffs) n/a
2 ccsbBroad304_11704 pLX_304 0% 15.7% 15.2% V5 (many diffs) n/a
3 TRCN0000474248 CCCCCCGAGCAAGAAGATTAATGC pLX_317 48.7% 15.7% 15.2% V5 (many diffs) n/a
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