Construct: ORF TRCN0000474250
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016782.1_s317c1
- Derived from:
- ccsbBroadEn_12068
- DNA Barcode:
- TATTTAGCACGCGCTCTCCGTACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MINDY2 (54629)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474250
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54629 | MINDY2 | MINDY lysine 48 deubiquitin... | XM_017022335.2 | 88.3% | 86.3% | (many diffs) |
| 2 | human | 54629 | MINDY2 | MINDY lysine 48 deubiquitin... | XM_017022334.2 | 66.8% | 64.2% | (many diffs) |
| 3 | human | 54629 | MINDY2 | MINDY lysine 48 deubiquitin... | XM_017022333.2 | 66.7% | 64% | (many diffs) |
| 4 | human | 54629 | MINDY2 | MINDY lysine 48 deubiquitin... | XM_017022332.2 | 64.6% | 62% | (many diffs) |
| 5 | human | 54629 | MINDY2 | MINDY lysine 48 deubiquitin... | XM_024449964.1 | 64.3% | 64.5% | 1122_1123insGGTG;1134_1758del |
| 6 | human | 54629 | MINDY2 | MINDY lysine 48 deubiquitin... | XM_011521690.2 | 64.1% | 64.3% | 1122_1123insGGTG;1134_1761del |
| 7 | human | 54629 | MINDY2 | MINDY lysine 48 deubiquitin... | XM_011521689.2 | 62.2% | 62.4% | 964_1017del;1176_1177insGGTG;1188_1815del |
| 8 | human | 54629 | MINDY2 | MINDY lysine 48 deubiquitin... | NM_001040453.3 | 60.7% | 60.9% | 1021_1122del;1224_1225insGGTG;1236_1860del |
| 9 | human | 54629 | MINDY2 | MINDY lysine 48 deubiquitin... | NM_001040450.3 | 60.6% | 60.8% | 1021_1122del;1224_1225insGGTG;1236_1863del |
| 10 | human | 54629 | MINDY2 | MINDY lysine 48 deubiquitin... | XR_001751324.2 | 59.2% | (many diffs) | |
| 11 | human | 54629 | MINDY2 | MINDY lysine 48 deubiquitin... | XM_011521688.2 | 59% | 59.2% | (many diffs) |
| 12 | human | 54629 | MINDY2 | MINDY lysine 48 deubiquitin... | XM_011521687.2 | 58.9% | 59.1% | (many diffs) |
| 13 | human | 54629 | MINDY2 | MINDY lysine 48 deubiquitin... | XR_001751323.2 | 58.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1203
- ORF length:
- 1137
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gagcagcccc gagagcctgc agccgctaga acacggggtg gcggccgggc 121 cagcgtcagg gacaggttct tcgcaggaag ggctacagga gaccaggctc gccgctggtg 181 atggtcctgg ggtatgggcg gcggagacca gcggcgggaa tgggctgggg gcggcggccg 241 ccaggaggag cctcccggac tcggcttctc ccgcgggctc tcctgaggtt cccggaccct 301 gcagctcctc cgcgggtttg gacttgaagg acagtggttt ggagagtcct gctgccgccg 361 aggcgcctct gagagggcag tacaaggtga ccgcctcccc ggagacagcc gtggccggag 421 tgggtcatga gttgggtacc gccggagacg cgggagcccg cccggatctc gccggcacct 481 gccaagcaga actgaccgcc gccggctccg aagagcccag cagcgccggc ggcctcagca 541 gcagttgcag cgacccgagc cctcctgggg aatctccgag cctggactct ctggagtcgt 601 tctctaacct gcattctttt cccagtagct gcgagttcaa tagtgaggag ggagcggaga 661 acagggtccc tgaggaggag gagggcgcgg cggtgttgcc cggggctgtt cctctgtgca 721 aggaggagga gggggaggag accgctcagg tgctggcggc ctccaaggaa cgcttcccgg 781 gacaatctgt gtatcacatc aagtggatCC AGTGGAAGGA AGAGAACACA CCCATCATCA 841 CCCAGAATGA GAACGGACCC TGCCCCTTGC TGGCCATCCT CAATGTTTTG CTCCTGGCCT 901 GGAAGGTGAA ACTTCCACCG ATGATGGAAA TCATAACTGC TGAGCAGCTG ATGGAATATT 961 TAGGAGATTA CATGCTTGAT GCAAAGCCAA AAGAAATTTC AGAAATTCAA CGTTTAAATT 1021 ATGAACAGAA TATGAGTGAT GCCATGGCAA TTTTGCACAA ACTACAGACA GGCCTGGATG 1081 TAAATATTGA TGACATTGTA AAAGCTGTTG GTAACTGCAG CTACAACCAA CTAGTGGAGA 1141 AGATCATCTC TTGTAAACAG TCAGACAATA GTGAGCTGGT TAGTGAAGGT GGGCTTTGTA 1201 GCTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1261 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1321 GGCTTTATAT ATCTTGTGGA AAGGACGATA TTTAGCACGC GCTCTCCGTA CTACGCGTTA 1381 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt