Construct: ORF TRCN0000474257
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006749.1_s317c1
- Derived from:
- ccsbBroadEn_11795
- DNA Barcode:
- CTCTCACCCCTGCTGTGTTTGGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NECAP1 (25977)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474257
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25977 | NECAP1 | NECAP endocytosis associated 1 | NM_015509.4 | 48.3% | 48.3% | 1_426del |
| 2 | human | 25977 | NECAP1 | NECAP endocytosis associated 1 | NR_024260.1 | 15.8% | 1_422del;822_2518del | |
| 3 | mouse | 67602 | Necap1 | NECAP endocytosis associated 1 | NM_026267.2 | 43% | 43.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 465
- ORF length:
- 399
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tgctcgtcct aagttggatc tgggcTTCAA GGAAGGACAA ACCATCAAGT 121 TGTGTATCGG GAACATTACA AACAAGAAAG GAGGTGCTTC TAAGCCCAGG ACTGCAAGGG 181 GTGGGGGTCT GAGCTTACTC CCACCCCCGC CAGGAGGCAA AGTCACTATT CCCCCACCAT 241 CCTCCTCAGT TGCCATCAGC AATCATGTCA CCCCACCACC CATTCCGAAA TCTAACCATG 301 GAGGCAGTGA TGCAGATATC CTTTTAGATT TGGATTCTCC TGCTCCTGTC ACGACACCAG 361 CACCAACTCC AGTTTCTGTA AGCAATGACT TGTGGGGAGA CTTCAGCACT GCCTCCAGCT 421 CTGTTCCAAA CCAGGCACCA CAGCCATCCA ACTGGGTCCA GTTCTGCCCA ACTTTCTTGT 481 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 541 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 601 GAAAGGACGA CTCTCACCCC TGCTGTGTTT GGCCACGCGT TAAGTCgaca atcaacctct 661 ggattacaaa atttgtgaaa gatt