Construct: ORF TRCN0000474285
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004137.1_s317c1
- Derived from:
- ccsbBroadEn_14345
- DNA Barcode:
- ACTCTCGTCCCGACTAAGGTTCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NAPRT (93100)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474285
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 93100 | NAPRT | nicotinate phosphoribosyltr... | NM_001363146.1 | 99.6% | 99.6% | 13G>C;108G>A;229C>T |
| 2 | human | 93100 | NAPRT | nicotinate phosphoribosyltr... | XM_017013979.2 | 79.4% | 72.2% | (many diffs) |
| 3 | human | 93100 | NAPRT | nicotinate phosphoribosyltr... | XM_024447333.1 | 72.4% | 65.5% | (many diffs) |
| 4 | human | 93100 | NAPRT | nicotinate phosphoribosyltr... | NM_145201.6 | 57.4% | 57.4% | (many diffs) |
| 5 | human | 93100 | NAPRT | nicotinate phosphoribosyltr... | NM_001286829.2 | 55% | 55% | (many diffs) |
| 6 | human | 93100 | NAPRT | nicotinate phosphoribosyltr... | NM_001363145.1 | 52.4% | 52.4% | (many diffs) |
| 7 | human | 93100 | NAPRT | nicotinate phosphoribosyltr... | XM_017013976.2 | 50.5% | 50.5% | (many diffs) |
| 8 | human | 93100 | NAPRT | nicotinate phosphoribosyltr... | XM_024447332.1 | 49.1% | 44% | (many diffs) |
| 9 | human | 93100 | NAPRT | nicotinate phosphoribosyltr... | XM_017013977.2 | 45.7% | 41.9% | (many diffs) |
| 10 | human | 93100 | NAPRT | nicotinate phosphoribosyltr... | XM_017013975.2 | 44.7% | 41.4% | (many diffs) |
| 11 | human | 93100 | NAPRT | nicotinate phosphoribosyltr... | XM_017013978.2 | 40.2% | 36.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 996
- ORF length:
- 930
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ggcgccacca gctggtgagg gccctggggt ggacctggcg gccaaagccc 121 aggtgtggct ggagcaggtg tgtgcccacc tggggctggg ggtgcaggag ccacatccag 181 gcgagcgggc agcctttgtg gcctatgcct tggcttttcc ccgggccttc cagggcctcc 241 tggacaccta cagcgtgtgg aggagtggtc tccccaactt cctagcagtc gccttggccc 301 tgggagagct gggctaccgg gcagtgggcg tgaggctgga cagtggtgac ctgctacagc 361 aggctcagga gatccgcaag gtcttccgag ctgctgcagc ccagttccag gtgccctggc 421 tggagtcagt cctcatcgta gtcagcaaca acattgacga ggaggcgctg gcccgactgg 481 cccaggaggg cagtgaggtg aatgtcattg gcattggcac cagtgtggtc acctgccccc 541 aacagccttc ccTGGGTGGC GTCTATAAGC TGGTGGCCGT GGGGGGCCAG CCACGAATGA 601 AGCTGACCGA GGACCCCGAG AAGCAGACGT TGCCTGGGAG CAAGGCTGCT TTCCGGCTCC 661 TGGGCTCTGA CGGGTCTCCA CTCATGGACA TGCTGCAGTT AGCAGAAGAG CCAGTGCCAC 721 AGGCTGGGCA GGAGCTGAGG GTGTGGCCTC CAGGGGCCCA GGAGCCCTGC ACCGTGAGGC 781 CAGCCCAGGT GGAGCCACTA CTGCGGCTCT GCCTCCAGCA GGGACAGCTG TGTGAGCCGC 841 TCCCATCCCT GGCAGAGTCT AGAGCCTTGG CCCAGCTGTC CCTGAGCCGA CTCAGCCCTG 901 AGCACAGGCG GCTGCGGAGC CCTGCACAGT ACCAGGTGGT GCTGTCCGAG AGGCTGCAGG 961 CCCTGGTGAA CAGTCTGTGT GCGGGGCAGT CCCCCTGCCC AACTTTCTTG TACAAAGTGG 1021 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1081 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1141 AACTCTCGTC CCGACTAAGG TTCTTACGCG TTAAGTCgac aatcaacctc tggattacaa 1201 aatttgtgaa agatt