Construct: ORF TRCN0000474286
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015402.1_s317c1
- Derived from:
- ccsbBroadEn_02654
- DNA Barcode:
- GCCTCAAACTTCACCCGTCTTGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDCD10 (11235)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474286
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11235 | PDCD10 | programmed cell death 10 | NM_007217.4 | 100% | 100% | |
| 2 | human | 11235 | PDCD10 | programmed cell death 10 | NM_145859.1 | 100% | 100% | |
| 3 | human | 11235 | PDCD10 | programmed cell death 10 | NM_145860.1 | 100% | 100% | |
| 4 | human | 11235 | PDCD10 | programmed cell death 10 | XM_005247086.5 | 100% | 100% | |
| 5 | human | 11235 | PDCD10 | programmed cell death 10 | XM_005247087.5 | 100% | 100% | |
| 6 | human | 11235 | PDCD10 | programmed cell death 10 | XM_005247088.4 | 100% | 100% | |
| 7 | human | 11235 | PDCD10 | programmed cell death 10 | XM_006713485.4 | 100% | 100% | |
| 8 | human | 11235 | PDCD10 | programmed cell death 10 | XM_011512368.3 | 100% | 100% | |
| 9 | human | 11235 | PDCD10 | programmed cell death 10 | XM_011512369.3 | 100% | 100% | |
| 10 | human | 11235 | PDCD10 | programmed cell death 10 | XM_017005644.2 | 100% | 100% | |
| 11 | human | 11235 | PDCD10 | programmed cell death 10 | XM_017005645.2 | 70.2% | 70.2% | 0_1ins189 |
| 12 | human | 11235 | PDCD10 | programmed cell death 10 | XM_024453329.1 | 70.2% | 70.2% | 0_1ins189 |
| 13 | human | 11235 | PDCD10 | programmed cell death 10 | XM_024453330.1 | 70.2% | 70.2% | 0_1ins189 |
| 14 | human | 11235 | PDCD10 | programmed cell death 10 | XM_024453331.1 | 70.2% | 70.2% | 0_1ins189 |
| 15 | mouse | 56426 | Pdcd10 | programmed cell death 10 | NM_019745.4 | 94.8% | 99.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 702
- ORF length:
- 636
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gatgacaatg gaagagatga agaatgaagc tgagaccaca tccatggttt 121 ctatgcccct ctatgcagtc atgtatcctg tgtttaatga gctagaacga gtaaatctgt 181 ctgcagccca gacactgaga gccgctttca tcaaggctga aaaagaaaat ccaggtctca 241 cacaagacat cattatgaaa attttagaga aaaaaagcgt ggaagttaac ttcacggagt 301 cccTTCTTCG TATGGCAGCT GATGATGTAG AAGAGTATAT GATTGAACGA CCAGAGCCAG 361 AATTCCAAGA CCTAAACGAA AAGGCACGAG CACTTAAACA AATTCTCAGT AAGATCCCAG 421 ATGAGATCAA TGACAGAGTG AGGTTTCTGC AGACAATCAA GGATATAGCT AGTGCAATAA 481 AAGAACTTCT TGATACAGTG AATAATGTCT TCAAGAAATA TCAATACCAG AACCGCAGGG 541 CACTTGAACA CCAAAAGAAA GAATTTGTAA AGTACTCCAA AAGTTTCAGT GATACTCTGA 601 AAACGTATTT TAAAGATGGC AAGGCAATAA ATGTGTTCGT AAGTGCCAAC CGACTAATTC 661 ATCAAACCAA CTTAATACTT CAGACCTTCA AAACTGTGGC CTGCCCAACT TTCTTGTACA 721 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 781 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 841 AGGACGAGCC TCAAACTTCA CCCGTCTTGT GACGCGTTAA GTCgacaatc aacctctgga 901 ttacaaaatt tgtgaaagat t