Construct: ORF TRCN0000474287
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008711.1_s317c1
- Derived from:
- ccsbBroadEn_03725
- DNA Barcode:
- TTCAAGCATATGGTAGAACCTCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNAJC12 (56521)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474287
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56521 | DNAJC12 | DnaJ heat shock protein fam... | NM_021800.3 | 100% | 100% | |
| 2 | human | 56521 | DNAJC12 | DnaJ heat shock protein fam... | XM_017016431.1 | 58.5% | 58.5% | 0_1ins246 |
| 3 | human | 56521 | DNAJC12 | DnaJ heat shock protein fam... | XM_017016432.2 | 58.5% | 58.5% | 0_1ins246 |
| 4 | human | 56521 | DNAJC12 | DnaJ heat shock protein fam... | NM_201262.1 | 51% | 49.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 660
- ORF length:
- 594
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tgcaatactg aattacaggt cagaagatac tgaagattac tacacattac 121 tgggatgtga tgaactatct tcggttgaac aaatcctggc agaatttaaa gtcagagctc 181 tggaatgtca cccagacaag catcctGAAA ACCCCAAAGC TGTGGAGACT TTTCAGAAAC 241 TGCAGAAGGC AAAGGAGATT CTGACCAATG AAGAGAGTCG AGCCCGCTAT GACCACTGGC 301 GAAGGAGCCA GATGTCGATG CCATTCCAGC AGTGGGAAGC TTTGAATGAC TCAGTGAAGA 361 CGTCAATGCA CTGGGTTGTC AGAGGTAAAA AAGACCTGAT GCTGGAAGAA TCTGACAAGA 421 CTCATACCAC CAAGATGGAA AATGAGGAAT GTAATGAGCA AAGAGAAAGA AAGAAAGAGG 481 AGCTGGCTTC AACCGCAGAG AAAACGGAGC AGAAAGAACC CAAGCCCCTA GAGAAGTCAG 541 TCTCCCCGCA AAATTCAGAT TCTTCAGGTT TTGCAGATGT GAATGGTTGG CACCTTCGTT 601 TCCGCTGGTC CAAGGATGCT CCCTCAGAAC TCCTGAGGAA GTTCAGAAAC TATGAAATAT 661 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 721 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAAACTTG AAAGTATTTC GATTTCTTGG 781 CTTTATATAT CTTGTGGAAA GGACGATTCA AGCATATGGT AGAACCTCGT ACGCGTTAAG 841 TCgacaatca acctctggat tacaaaattt gtgaaagatt