Construct: ORF TRCN0000474291
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012921.2_s317c1
- Derived from:
- ccsbBroadEn_15143
- DNA Barcode:
- AACACGCGGGGAGCCTCGGCCTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STK33 (65975)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474291
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001289061.2 | 99.5% | 99.2% | (many diffs) |
| 2 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352387.2 | 99.5% | 99.2% | (many diffs) |
| 3 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352388.2 | 99.5% | 99.2% | (many diffs) |
| 4 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352389.2 | 99.5% | 99.2% | (many diffs) |
| 5 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352390.2 | 99.5% | 99.2% | (many diffs) |
| 6 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352391.2 | 99.5% | 99.2% | (many diffs) |
| 7 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352392.2 | 99.5% | 99.2% | (many diffs) |
| 8 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352393.2 | 99.5% | 99.2% | (many diffs) |
| 9 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_030906.4 | 99.5% | 99.2% | (many diffs) |
| 10 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_011520290.2 | 99.5% | 99.2% | (many diffs) |
| 11 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_011520295.2 | 99.5% | 99.2% | (many diffs) |
| 12 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018144.2 | 99.5% | 99.2% | (many diffs) |
| 13 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018145.1 | 99.5% | 99.2% | (many diffs) |
| 14 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018146.1 | 99.5% | 99.2% | (many diffs) |
| 15 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018147.1 | 99.5% | 99.2% | (many diffs) |
| 16 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018148.1 | 99.5% | 99.2% | (many diffs) |
| 17 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018152.1 | 99.5% | 99.2% | (many diffs) |
| 18 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018153.2 | 99.5% | 99.2% | (many diffs) |
| 19 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018154.1 | 99.5% | 99.2% | (many diffs) |
| 20 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018155.2 | 99.5% | 99.2% | (many diffs) |
| 21 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018156.1 | 99.5% | 99.2% | (many diffs) |
| 22 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001289058.2 | 91.5% | 91.2% | (many diffs) |
| 23 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352394.2 | 91.5% | 91.2% | (many diffs) |
| 24 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352395.2 | 91.5% | 91.2% | (many diffs) |
| 25 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_011520297.2 | 91.5% | 91.2% | (many diffs) |
| 26 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018157.1 | 91.5% | 91.2% | (many diffs) |
| 27 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352399.2 | 86.8% | 86.7% | (many diffs) |
| 28 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018159.1 | 86.8% | 86.7% | (many diffs) |
| 29 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018161.1 | 78.3% | 72.5% | (many diffs) |
| 30 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352396.2 | 70.4% | 64.7% | (many diffs) |
| 31 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352397.2 | 70.4% | 64.7% | (many diffs) |
| 32 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352398.2 | 70.4% | 64.7% | (many diffs) |
| 33 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001289059.2 | 63.2% | 62.8% | (many diffs) |
| 34 | human | 65975 | STK33 | serine/threonine kinase 33 | XR_001747938.1 | 34.3% | (many diffs) | |
| 35 | human | 65975 | STK33 | serine/threonine kinase 33 | XR_001747939.1 | 25.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1608
- ORF length:
- 1542
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgatagtggc ttagataaaa aatccacaaa atgccccgac tgttcatctg 121 cttctcagaa agatgtactt tgtgtatgtt ccagcaaaac aagggttcct ccagttttgg 181 tggtggaaat gtcacagaca tcaagcattg gtagtgcaga atctttaatt tcactggaga 241 gaaaaaaaga aaaaaatatc aacagagata taacctccag gaaagatttg ccctcaagaa 301 cctcaaatgt agagagaaaa gcatctcagc aacaatgggg tcggggcaac tttacagaag 361 gaaaagttcc tcacataagg attgagaatg gagctgctat tgaggaaatc tatacctttg 421 gaagaatatt gggaaaaggg agctttggaa tagtcattga agctacagac aaggaaacag 481 aaacgaagtg ggcaattaaa aaagtgaaca aagaaaaggc tggaagctcc gctgtgaagt 541 tacttgaacg agaggtgaac attctgaaaa gtgtaaaaca tgaacacatc atacatctgg 601 aacaagtatt tgaaacgcca aagaaaatgt accttgtgat ggagctttgt gaggatggag 661 aactcaaaga aattctggat aggaaagggc atttctcaga gaatgagaca aggtggatca 721 ttcaaagtct cgcatcagct atagcatatc ttcacaataa tgatattgta catagggatc 781 tgaaactgga aaatataatg gttaaaagca gtcttattga tgataacaat gaaataaact 841 taaacataaa ggtgactgat tttggcttag cggtgaagaa gcaaagtagg agtgaagcca 901 tgctgcaggc cacatgtggg actcctatct atatggcccc tgaagttatc agtgcccacg 961 actatagcca gcagtgtgac atttggagca taggcgtcgt aatgtacatg ttattacgtg 1021 gagaaccacc ctttttggca agctcagaag agaagctttt tgagttaata agaaaaggag 1081 aaCTACATTT TGAAAATGCA GTCTGGAATT CCATAAGTGA CTGTGCTAAA AGTGTTTTGA 1141 AACAACTTAT GAAAGTAGAT CCTGCTCACA GAATCACAGC TAAGGAACTA CTAGATAACC 1201 AGTGGTTAAC AGGCAATAAA CTTTCTTCGG TGAGACCAAC CAATGTATTA GAGATGATGA 1261 AGGAATGGAA AAATAACCCA GAAAGTGTTG AGGAAAACAC AACAGAAGAG AAGAATAAGC 1321 CGTCCACTGA AGAAAAGTTG AAAAGTTACC AACCCTGGGG AAATGTCCCT GAGACCAATT 1381 ACACTTCAGA TGAAGAGGAG GAAAAACAGT CTACTACTTA TGAAAAGCAA TTTCCTGCAA 1441 CCAGTAAGGA CAACTTTGAT ATGTGCAGTT CAAGTTTCAC ATCTAGCAAA CTCCTTCCAG 1501 CTGAAATCAA GGGAGAAATG GAGAAAACCC CTGTGACTCC AAGCCAAGGA ACAGCAACCA 1561 AGTACCCTGC TAAATCCGGC GCCCTGTCCA GAACCAAAAA GGAACTCTAC CCAACTTTCT 1621 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1681 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1741 GTGGAAAGGA CGAAACACGC GGGGAGCCTC GGCCTTAACG CGTTAAGTCg acaatcaacc 1801 tctggattac aaaatttgtg aaagatt