Transcript: Human XM_017018146.1

PREDICTED: Homo sapiens serine/threonine kinase 33 (STK33), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
STK33 (65975)
Length:
2881
CDS:
697..2241

Additional Resources:

NCBI RefSeq record:
XM_017018146.1
NBCI Gene record:
STK33 (65975)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147351 ACACTGCTGGCTATAGTCGT pXPR_003 GGG 895 58% 12 1.1772 STK33 STK33 76985
2 BRDN0001148596 CCTCACAAAGCTCCATCACA pXPR_003 AGG 572 37% 9 0.2664 STK33 STK33 76987
3 BRDN0001149120 TGTGAAGTTACTTGAACGAG pXPR_003 AGG 484 31% 8 -0.1278 STK33 STK33 76986
4 BRDN0001149402 GGTCGGGGCAACTTTACAGA pXPR_003 AGG 290 19% 6 -0.1806 STK33 STK33 76984
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017018146.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000229974 GAAACGAAGTGGGCAATTAAA pLKO_005 1111 CDS 100% 15.000 21.000 N STK33 n/a
2 TRCN0000229977 GTCGTAATGTACATGTTATTA pLKO_005 1627 CDS 100% 15.000 21.000 N STK33 n/a
3 TRCN0000382487 ATAAACTTGCCATACGTATTA pLKO_005 2683 3UTR 100% 13.200 18.480 N STK33 n/a
4 TRCN0000382192 CAACCAAGTACCCTGCTAAAT pLKO_005 2186 CDS 100% 13.200 18.480 N STK33 n/a
5 TRCN0000195277 CGTCGTAATGTACATGTTATT pLKO.1 1626 CDS 100% 13.200 18.480 N STK33 n/a
6 TRCN0000229976 TTATCAGTGCCCACGACTATA pLKO_005 1577 CDS 100% 13.200 18.480 N STK33 n/a
7 TRCN0000229975 CTCGCATCAGCTATAGCATAT pLKO_005 1360 CDS 100% 10.800 15.120 N STK33 n/a
8 TRCN0000196915 GCCATACGTATTACAGCAGAA pLKO.1 2691 3UTR 100% 4.050 5.670 N STK33 n/a
9 TRCN0000218466 ATGTTTAGGCACAGCTATTTA pLKO_005 2573 3UTR 100% 15.000 12.000 N STK33 n/a
10 TRCN0000382280 GTAATGGAGGTGCCAAATAAT pLKO_005 2620 3UTR 100% 15.000 12.000 N STK33 n/a
11 TRCN0000196819 GCCAAATAATTATGTGCACTT pLKO.1 2631 3UTR 100% 4.050 3.240 N STK33 n/a
12 TRCN0000381374 TAAGCTGCACATGGCATTAAA pLKO_005 2418 3UTR 100% 15.000 10.500 N STK33 n/a
13 TRCN0000379525 AGCAGTCTTATTGATGATAAC pLKO_005 1438 CDS 100% 10.800 7.560 N STK33 n/a
14 TRCN0000380970 ATGAGACAAGGTGGATCATTC pLKO_005 1334 CDS 100% 10.800 7.560 N STK33 n/a
15 TRCN0000196452 GCTAAGGAACTACTAGATAAC pLKO.1 1810 CDS 100% 10.800 7.560 N STK33 n/a
16 TRCN0000382463 TTGAACGAGAGGTGAACATTC pLKO_005 1175 CDS 100% 10.800 7.560 N STK33 n/a
17 TRCN0000002080 CCCTCAAGAACCTCAAATGTA pLKO.1 922 CDS 100% 5.625 3.938 N STK33 n/a
18 TRCN0000002077 CCAAGGAACAGCAACCAAGTA pLKO.1 2175 CDS 100% 4.950 3.465 N STK33 n/a
19 TRCN0000002078 GCAGTTCAAGTTTCACATCTA pLKO.1 2096 CDS 100% 4.950 3.465 N STK33 n/a
20 TRCN0000002081 CTTGCCATTAACTTGCTGCTA pLKO.1 2812 3UTR 100% 2.640 1.848 N STK33 n/a
21 TRCN0000002079 GAACACATCATACATCTGGAA pLKO.1 1213 CDS 100% 2.640 1.848 N STK33 n/a
22 TRCN0000221229 GCAGTGTGACATTTGGAGCAT pLKO.1 1602 CDS 100% 2.640 1.848 N Stk33 n/a
23 TRCN0000380779 GGAGCTTTGGAATAGTCATTG pLKO_005 1070 CDS 100% 10.800 6.480 N STK33 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017018146.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489906 ATAATGCAGGTGGGCCGATGTCAA pLX_317 25.7% 100% 100% V5 (not translated due to prior stop codon) n/a
2 ccsbBroadEn_12518 pDONR223 100% 99.6% 99.4% None (many diffs) n/a
3 ccsbBroad304_12518 pLX_304 0% 99.6% 99.4% V5 (many diffs) n/a
4 TRCN0000473158 TACTAGCTGGGAAAGTGCGCCAGC pLX_317 30.7% 99.6% 99.4% V5 (many diffs) n/a
5 ccsbBroadEn_15143 pDONR223 0% 99.6% 99.4% None (many diffs) n/a
6 ccsbBroad304_15143 pLX_304 0% 99.6% 99.4% V5 (many diffs) n/a
7 TRCN0000474291 AACACGCGGGGAGCCTCGGCCTTA pLX_317 34.3% 99.5% 99.2% V5 (many diffs) n/a
8 TRCN0000489525 CCCTGATCTCATCTGTTTTTCAGT pLX_317 25.9% 99.6% 99.4% V5 (not translated due to prior stop codon) (many diffs) n/a
9 TRCN0000488757 GGTGGCTATACACACTGAAACTCT pLX_317 22.4% 99.5% 99.2% V5 (many diffs) n/a
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