Construct: ORF TRCN0000474298
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015380.1_s317c1
- Derived from:
- ccsbBroadEn_02911
- DNA Barcode:
- GGTCTTTTAATACGGATCGAGTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLK13 (26085)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474298
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26085 | KLK13 | kallikrein related peptidas... | NM_015596.3 | 100% | 100% | |
| 2 | human | 26085 | KLK13 | kallikrein related peptidas... | XR_935788.1 | 85.8% | 1_39del;547_626del;951_968del | |
| 3 | human | 26085 | KLK13 | kallikrein related peptidas... | NM_001348177.2 | 73.6% | 73.6% | 288_289ins219 |
| 4 | human | 26085 | KLK13 | kallikrein related peptidas... | NM_001348178.2 | 43.1% | 45.1% | (many diffs) |
| 5 | human | 26085 | KLK13 | kallikrein related peptidas... | NR_145464.2 | 38.1% | (many diffs) | |
| 6 | human | 26085 | KLK13 | kallikrein related peptidas... | NR_145466.2 | 37.5% | (many diffs) | |
| 7 | human | 26085 | KLK13 | kallikrein related peptidas... | NR_145463.2 | 34% | 1_25del;321_322ins211;646_1609del | |
| 8 | human | 26085 | KLK13 | kallikrein related peptidas... | NR_145465.2 | 29.5% | (many diffs) | |
| 9 | human | 26085 | KLK13 | kallikrein related peptidas... | NR_145467.2 | 28.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 897
- ORF length:
- 831
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg gcccctggcc ctagtgatcg cctccctgac cttggccttg tcaggaggtg 121 tctcccagga gtcttccaag gttctcaaca ccaatgggac cagtgggttt ctcccaggtg 181 gctacacctg cttcccccac tctcagccct ggcaggctgc cctactagtg caagggcggc 241 tactctgtgg gggagtcctg gtccacccca aatgggtcct cactgccgca cactgtctaa 301 aggaggggct caaagtttac ctaggcaagc acgccctagg gcgtgtggaa gctggtgagc 361 aggtgaggga agttgtccac tctatccccc accctgaata ccggagaagc cccacccacc 421 tgaaccacga ccatgacatc atgcttctgg agctgcagtc cccggtccag ctcacaggct 481 acatccaaac cctgcccctt tcccacaaca accgccTAAC CCCTGGCACC ACCTGTCGGG 541 TGTCTGGCTG GGGCACCACC ACCAGCCCCC AGGTGAATTA CCCCAAAACT CTACAATGTG 601 CCAACATCCA ACTTCGCTCA GATGAGGAGT GTCGTCAAGT CTACCCAGGA AAGATCACTG 661 ACAACATGTT GTGTGCCGGC ACAAAAGAGG GTGGCAAAGA CTCCTGTGAG GGTGACTCTG 721 GGGGCCCCCT GGTCTGTAAC AGAACACTGT ATGGCATCGT CTCCTGGGGA GACTTCCCAT 781 GTGGGCAACC TGACCGGCCT GGTGTCTACA CCCGTGTCTC AAGATACGTC CTGTGGATCC 841 GTGAAACAAT CCGAAAATAT GAAACCCAGC AGCAAAAATG GTTGAAGGGC CCACAATACC 901 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 961 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1021 ATATATCTTG TGGAAAGGAC GAGGTCTTTT AATACGGATC GAGTATACGC GTTAAGTCga 1081 caatcaacct ctggattaca aaatttgtga aagatt