Construct: ORF TRCN0000474302

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006426.1_s317c1
Derived from:
ccsbBroadEn_07445
DNA Barcode:
TAGCACATGCCGAATGAATTGTGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MACROH2A1 (9555)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474302
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9555 MACROH2A1 macroH2A.1 histone NM_138610.2 99.9% 99.7% 1108G>C
2 human 9555 MACROH2A1 macroH2A.1 histone XM_011543729.3 99.9% 99.7% 1108G>C
3 human 9555 MACROH2A1 macroH2A.1 histone NM_001040158.1 99.6% 99.4% 474_475insAAG;1105G>C
4 human 9555 MACROH2A1 macroH2A.1 histone NM_004893.3 99.6% 99.4% 474_475insAAG;1105G>C
5 human 9555 MACROH2A1 macroH2A.1 histone XM_011543730.3 99.6% 99.4% 474_475insAAG;1105G>C
6 human 9555 MACROH2A1 macroH2A.1 histone NM_138609.2 94.2% 93% (many diffs)
7 human 9555 MACROH2A1 macroH2A.1 histone XM_005272132.2 93.9% 92.7% (many diffs)
8 human 9555 MACROH2A1 macroH2A.1 histone XM_011543731.2 72.5% 68.8% 776_777ins175;810_811ins131
9 human 9555 MACROH2A1 macroH2A.1 histone XM_011543732.2 72.3% 68.6% 474_475insAAG;773_774ins175;807_808ins131
10 human 9555 MACROH2A1 macroH2A.1 histone XM_024446263.1 66.9% 62% (many diffs)
11 human 9555 MACROH2A1 macroH2A.1 histone XM_011543733.3 63.3% 55.5% (many diffs)
12 human 9555 MACROH2A1 macroH2A.1 histone XM_017010067.2 63% 55.3% (many diffs)
13 human 9555 MACROH2A1 macroH2A.1 histone XM_017010068.2 62.8% 61% (many diffs)
14 human 9555 MACROH2A1 macroH2A.1 histone XM_024446264.1 59.6% 53.1% 474_475insAAG;583_584ins100;666_667ins347
15 human 9555 MACROH2A1 macroH2A.1 histone XR_948308.2 54.8% (many diffs)
16 human 9555 MACROH2A1 macroH2A.1 histone XR_948307.2 54.7% (many diffs)
17 human 9555 MACROH2A1 macroH2A.1 histone XR_948309.2 54.5% (many diffs)
18 human 9555 MACROH2A1 macroH2A.1 histone XR_001742356.2 54.1% (many diffs)
19 human 9555 MACROH2A1 macroH2A.1 histone XR_001742358.2 54% (many diffs)
20 human 9555 MACROH2A1 macroH2A.1 histone XM_005272134.4 53.2% 52.6% (many diffs)
21 human 9555 MACROH2A1 macroH2A.1 histone XR_948306.3 53.2% (many diffs)
22 human 9555 MACROH2A1 macroH2A.1 histone XR_001742353.2 53% (many diffs)
23 human 9555 MACROH2A1 macroH2A.1 histone XM_005272135.4 52.9% 52.4% (many diffs)
24 human 9555 MACROH2A1 macroH2A.1 histone XM_011543734.3 52.6% 52.6% 588_589ins528
25 human 9555 MACROH2A1 macroH2A.1 histone XM_011543735.3 52.4% 52.4% 474_475insAAG;585_586ins528
26 human 9555 MACROH2A1 macroH2A.1 histone XR_948310.2 52.1% (many diffs)
27 human 9555 MACROH2A1 macroH2A.1 histone XR_948311.2 51.9% (many diffs)
28 human 9555 MACROH2A1 macroH2A.1 histone XR_001742359.2 51% (many diffs)
29 human 9555 MACROH2A1 macroH2A.1 histone XR_001742354.2 50.4% (many diffs)
30 human 9555 MACROH2A1 macroH2A.1 histone XR_001742355.2 50.3% (many diffs)
31 human 9555 MACROH2A1 macroH2A.1 histone XR_001742357.2 50.3% (many diffs)
32 mouse 26914 Macroh2a1 macroH2A.1 histone NM_012015.2 90.5% 98.1% (many diffs)
33 mouse 26914 Macroh2a1 macroH2A.1 histone NM_001159513.1 90.3% 97.8% (many diffs)
34 mouse 26914 Macroh2a1 macroH2A.1 histone NM_001159514.1 84.7% 91.3% (many diffs)
35 mouse 26914 Macroh2a1 macroH2A.1 histone NM_001159515.1 84.4% 91.1% (many diffs)
36 mouse 26914 Macroh2a1 macroH2A.1 histone XM_006517257.3 47.5% 51.8% (many diffs)
37 mouse 26914 Macroh2a1 macroH2A.1 histone XR_001780785.1 47.5% (many diffs)
38 mouse 26914 Macroh2a1 macroH2A.1 histone XR_382440.2 47.4% (many diffs)
39 mouse 26914 Macroh2a1 macroH2A.1 histone XR_001780786.1 47.4% (many diffs)
40 mouse 26914 Macroh2a1 macroH2A.1 histone XR_003950458.1 47.3% (many diffs)
41 mouse 26914 Macroh2a1 macroH2A.1 histone XM_006517258.3 47.3% 51.6% (many diffs)
42 mouse 26914 Macroh2a1 macroH2A.1 histone XR_001780784.1 46.4% (many diffs)
43 mouse 26914 Macroh2a1 macroH2A.1 histone XR_003950457.1 46.3% (many diffs)
44 mouse 26914 Macroh2a1 macroH2A.1 histone XR_003950456.1 28.5% (many diffs)
45 mouse 26914 Macroh2a1 macroH2A.1 histone XR_003950455.1 28.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1182
ORF length:
1116
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc gagccgcggt gggaagaaga agtccaccaa gacgtccagg tctgccaaag
121caggagtcat ctttcccgtg gggcggatgc tgcggtacat caagaaaggc caccccaagt
181acaggattgg agtgggggca cccgtgtaca tggccgccgt cctggaatac ctgacagcgg
241agattctgga gctggctggc aatgcagcga gagacaacaa gaagggacgg gtcacacccc
301ggcacatcct gctggctgtg gccaatgatg aagagctgaa tcagctgcta aaaggagtca
361ccatagccag tgggggtgtg ttacccaaca tccaccccga gttgctagcg aagaagcggg
421gatccaaagg aaagttggaa gccatcatca caccaccccc agccaaaaag gccaagtctc
481catcccagaa gaagcctgta tctaaaaaag caggaggcaa gaaaggggcc cggaaatcca
541agaagaagca gggtgaagtc agtaaggcag ccagcgccga cagcacaacc gagggcacac
601ctgccgacgg cttcacagtc ctctccacca agagcctctt ccttggccag aagctgaacc
661ttattcacag tgaaatcagt aatttagccg gctttgaggt ggaggccata atcaatccta
721ccaatgctga cattgacctt aaagatgacc taggaaacac gctggagaag aaaggtggca
781aggagtttgt ggaagctgtc ctggaacTCC GGAAAAAGAA CGGGCCCTTG GAAGTAGCTG
841GAGCTGCTGT CAGCGCAGGC CATGGCCTGC CTGCCAAGTT TGTGATCCAC TGTAATAGTC
901CAGTTTGGGG TGCAGACAAG TGTGAAGAAC TTCTGGAAAA GACAGTGAAA AACTGCTTGG
961CCCTGGCTGA TGATAAGAAG CTGAAATCCA TTGCATTTCC ATCCATCGGC AGCGGCAGGA
1021ACGGTTTTCC AAAGCAGACA GCAGCTCAGC TGATTCTGAA GGCCATCTCC AGTTACTTCG
1081TGTCTACAAT GTCCTCTTCC ATCAAAACGG TGTACTTCGT GCTTTTTGAC AGCGAGAGTA
1141TAGGCATCTA TGTGCAGGAA ATGGCCAAGC TGCACGCCAA CTACCCAACT TTCTTGTACA
1201AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
1261AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
1321AGGACGATAG CACATGCCGA ATGAATTGTG CACGCGTTAA GTCgacaatc aacctctgga
1381ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)