Construct: ORF TRCN0000474331
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008375.1_s317c1
- Derived from:
- ccsbBroadEn_08680
- DNA Barcode:
- CGCGCAAGGTTAAGCCTCATGTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSMG2 (56984)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474331
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56984 | PSMG2 | proteasome assembly chapero... | NM_020232.5 | 99.8% | 100% | 303A>G |
| 2 | human | 56984 | PSMG2 | proteasome assembly chapero... | NM_147163.1 | 88.1% | 88.2% | 0_1ins93;210A>G |
| 3 | mouse | 107047 | Psmg2 | proteasome (prosome, macrop... | NM_134138.1 | 84.4% | 87.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 858
- ORF length:
- 792
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt cgttccctgc ggggagtcgg cccccgacct tgccggcttc accctcctaa 121 tgccagcagt atctgttgga aatgttggcc agcttgcaat ggatctgatt atttctacac 181 tgaatatgtc taagattggt tacttctata ccgattgtct tgtgccaatg gttggaaaca 241 atccatatgc gaccacagaa ggaaattcaa cagaacttag cataaatgct gaagtgtatt 301 cattgccttc aagaaagctg gtggctctac agttaagatc catttttatt aagtataaat 361 caaagccgtt ctgtgaaaaa ctgctttcct gggtgaaaag cagtggctgt gccagagtca 421 ttgttctttc aagcagtcat tcatatcagc gtaatgatct gcagcttcgt agtactccct 481 tccggtacct acttacaccT TCCATGCAAA AAAGTGTTCA AAATAAAATA AAGAGCCTTA 541 ACTGGGAAGA AATGGAAAAA AGCCGGTGCA TTCCTGAAAT AGATGATTCC GAGTTTTGTA 601 TCCGCATTCC GGGAGGAGGT ATCACAAAAA CACTCTATGA TGAAAGCTGT TCTAAAGAAA 661 TCCAAATGGC AGTTCTGCTG AAATTTGTTT CAGAAGGGGA CAACATCCCA GATGCATTAG 721 GTCTTGTTGA GTATCTTAAT GAGTGGCTTC AGATACTCAA ACCACTTAGC GATGACCCCA 781 CAGTATCTGC CTCACGGTGG AAAATACCAA GTTCTTGGAG ATTACTCTTT GGCAGTGGTC 841 TTCCCCCTGC ACTTTTCTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 901 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 961 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACGCGCAA GGTTAAGCCT 1021 CATGTAGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt