Construct: ORF TRCN0000474332
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003057.1_s317c1
- Derived from:
- ccsbBroadEn_16090
- DNA Barcode:
- AACAACTTCACCACCTTTAGTTCA
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AP1S3 (130340)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474332
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 130340 | AP1S3 | adaptor related protein com... | XM_011510600.3 | 91.5% | 91.6% | (many diffs) |
| 2 | human | 130340 | AP1S3 | adaptor related protein com... | NM_001039569.2 | 63.4% | 63.6% | (many diffs) |
| 3 | human | 130340 | AP1S3 | adaptor related protein com... | NR_110905.2 | 7.7% | 1_132del;432delT;445_4025del | |
| 4 | human | 130340 | AP1S3 | adaptor related protein com... | NR_110906.2 | 7% | (many diffs) | |
| 5 | mouse | 252903 | Ap1s3 | adaptor-related protein com... | NM_183027.2 | 56.6% | 59.7% | (many diffs) |
| 6 | mouse | 252903 | Ap1s3 | adaptor-related protein com... | XR_373542.1 | 17.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 402
- ORF length:
- 312
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGATACATTT CATATTGCTC TTCAGTCGAC 121 AAGGGAAATT ACGGCTACAG AAATGGTACA TCACTCTCCC TGATAAAGAG AGGAAGAAGA 181 TCACCCGGGA AATTGTTCAG ATTATTCTCT CCCGTGGTCA CAGGACAAGC AGTTTTGTTG 241 ACTGGAAGGA GCTAAAACTT GTTTATAAAA GGTATGCTAG TTTATATTTT TGCTGTGCAA 301 TAGAAAATCA GGACAATGAG CTCTTGACGC TAGAGATTGT GCATCGTTAC GTGGAGCTGC 361 TGGACAAATA TTTTGGAAAT ACTTGGCCTT TGCAAGAGCC TGCCCAACTT TCTTGTACAA 421 AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA 481 ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA 541 GGACGAAACA ACTTCACCAC CTTTAGTTCA ACGCGTTAAG TCgacaatca acctctggat 601 tacaaaattt gtgaaagatt