Construct: ORF TRCN0000474338
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008290.1_s317c1
- Derived from:
- ccsbBroadEn_09885
- DNA Barcode:
- ATCTTACGACCGAGCGGCCACCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NKAPL (222698)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474338
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 222698 | NKAPL | NFKB activating protein like | NM_001007531.3 | 99.8% | 99.5% | 287A>G;1193A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1272
- ORF length:
- 1206
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc cccagtatcc cggtccagct attccgagga catcgtgggc tctcggagaa 121 ggcgacgcag ctcctcgggg agcccaccat ccccgcagag cagatgttcc tcttgggatg 181 gctgttcccg ctctcactcc cgcggccgtg agggcctcag gcctccttgg agtgagttgg 241 acgtgggcgc tctttacccc tttagtcgct ctgggtcgcg agggcggctc ccaagattcc 301 gcaactacgc cttcgcgtcc tcctggtcga cctcgtatag tggatatcgc tgccatcgtc 361 actgctatgc agaagaacgg cagtcagcgg aagactacga gaaggaagag agccatcggc 421 agaggaggct gaaggagaga gagaggattg gggaattggg agcgcctgaa gtgtgggggc 481 cgtctccaaa gttccctcag ctagattctg acgaacatac cccagttgag gatgaagaag 541 aggtaacgca tcagaaaagc agcagttcag attccaactc ggaagaacat aggaaaaaga 601 agaccagtcg ttcaagaaac aagaaaaaaa gaaagaataa gtcgtctaaa agaaagcata 661 ggaaatattc tgatagtgac agtaactcag agtctgacac aaattctgac tctgatgatg 721 ataaaaagag agttaaagcc aagaagaaaa agaagaaaaa gaaacacaaa acaaagaaaa 781 agaagaataa gaaaaccaaa aaagaatcca gtgactcaag ctgtaaagac tcagaagagg 841 acttgTCAGA AGCTACCTGG ATGGAGCAGC CAAATGTGGC AGATACTATG GATTTAATAG 901 GGCCAGAAGC ACCTATAATA CATACCTCTC AAGATGAAAA ACCTTTGAAG TATGGCCATG 961 CTTTGCTTCC CGGTGAAGGT GCAGCTATGG CTGAGTATGT AAAAGCTGGA AAGCGAATCC 1021 CACGAAGAGG TGAAATTGGG TTGACAAGTG AAGAGATCGG TTCTTTTGAA TGCTCAGGTT 1081 ATGTCATGAG TGGTAGCAGG CATCGCAGAA TGGAGGCTGT ACGACTGCGT AAGGAGAACC 1141 AGATCTACAG TGCTGATGAG AAGAGAGCTC TTGCATCCTT TAACCAAGAA GAGAGACGAA 1201 AGAGAGAAAG TAAGATTTTA GCCAGTTTCC GAGAGATGGT GCACAAAAAG ACAAAAGGGA 1261 AAGATGACAA GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1321 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1381 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAATC TTACGACCGA GCGGCCACCG 1441 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t